Occurrence of entomopathogenic fungus Metarhizium anisopliae isolated from island bris and coastal soils of Terengganu Malaysia

Insyirah Ishak, . and Pong Kuan Kin, . and Wahizatul Afzan Azmi, . and Muhammad Shahrul Hafiz Elham, . and Lee Grace Ern Lin, . Occurrence of entomopathogenic fungus Metarhizium anisopliae isolated from island bris and coastal soils of Terengganu Malaysia. pp. 179-189. ISSN 1823-8556

Entomopathogenic Fungi (EPF) are environmental friendly biological control agent that can be used to control insect pests. These fungi can be found naturally from the soils of natural forest and agricultural areas. The main aims of this study were to isolate and determine the occurrence of EPF from soils of island BRIS (Beach Ridges Interspersed with Swales) and coastal areas in Terengganu Malaysia. The EPF were isolated from the soil samples by using insect baiting method with the larvae of mealworm Tenebrio molitor. Physico-chemical analyses of soil such as soil texture pH water content and nitrogen analysis were also carried out in this study. Metarhizium anisopliae was the only EPF species found from the soil samples of the island of Taman Tropika Kenyir (three isolates) and BRIS soil of Marang (four isolates). No EPF was found in the coastal soil samples of Tok Jembal Beach. Significantly larger spore size of M. anisopliae from island soil was recorded compared to BRIS soil (F5.816 p0.05). On Potato Dextrose Agar the colony of M. Anisopliae was whitish yellow and turned to dark green when matured; slow growing with floccose mycelium. Lower water content and pH value were significantly associated with the lower occurrence of EPF in the island and BRIS soils. Further research can be carried out on the pathogenicity of M. Anisopliae against insect pests.

Full text available from: Official URL: http://jssm.umt.edu.my/wp-content/uploads/sites/51...

Abstract

Entomopathogenic Fungi (EPF) are environmental friendly biological control agent that can be used to control insect pests. These fungi can be found naturally from the soils of natural forest and agricultural areas. The main aims of this study were to isolate and determine the occurrence of EPF from soils of island BRIS (Beach Ridges Interspersed with Swales) and coastal areas in Terengganu Malaysia. The EPF were isolated from the soil samples by using insect baiting method with the larvae of mealworm Tenebrio molitor. Physico-chemical analyses of soil such as soil texture pH water content and nitrogen analysis were also carried out in this study. Metarhizium anisopliae was the only EPF species found from the soil samples of the island of Taman Tropika Kenyir (three isolates) and BRIS soil of Marang (four isolates). No EPF was found in the coastal soil samples of Tok Jembal Beach. Significantly larger spore size of M. anisopliae from island soil was recorded compared to BRIS soil (F5.816 p0.05). On Potato Dextrose Agar the colony of M. Anisopliae was whitish yellow and turned to dark green when matured; slow growing with floccose mycelium. Lower water content and pH value were significantly associated with the lower occurrence of EPF in the island and BRIS soils. Further research can be carried out on the pathogenicity of M. Anisopliae against insect pests.

[error in script]

Item Type:	Article
AGROVOC Term:	Metarhizium anisopliae
AGROVOC Term:	Entomopathogenic fungi
AGROVOC Term:	Isolation of microorganisms
AGROVOC Term:	Soil
AGROVOC Term:	Islands
AGROVOC Term:	Beaches
AGROVOC Term:	Coastal area
AGROVOC Term:	Coastal soils
AGROVOC Term:	Species diversity
AGROVOC Term:	Physicochemical properties
Depositing User:	Ms. Suzila Mohamad Kasim
Last Modified:	24 Apr 2025 06:29
URI:	http://webagris.upm.edu.my/id/eprint/25002

Actions (login required)

View Item