Citation
Jeffrine Japning Rovie-Ryan, . and Salmah Yaakop, . and Siti Norsyuhada Kamaluddin, . and Wan Mohd Razi Idris, . and Sabapathy Dharmalingam, . and Badrul Munir Md-Zain, . Genetic identification of critically endangered orangutans in captivity. pp. 57-68. ISSN 1823-8556
Abstract
A large numerals of the orangutans genetic status in captivity is unknown to its exact thus complicates the translocation process to their true natural habitat. A study was carried out to identify 37 captive orangutans in Peninsular Malaysia at the species and subspecies level using mitochondrial DNA (mtDNA) displacement loop (D-loop) region DNA sequences. Orangutan genetic samples were provided by the Bukit Merah Orang Utan Island (BMOUI) Zoo Negara Zoo Taiping and the A Famosa. Total genomic DNA was extracted from both fecal and blood samples. Polymerase chain reaction (PCR) was performed using specific primers for the orang utan. Data analyses were carried out using distance and character based approaches namely the neighbor joining (NJ) maximum parsimony (MP) and Bayesian techniques. About 8 individuals were identified as Pongo pygmaeus pygmaeus 20 as P. p. morio 5 as P. p. wurmbii and 4 as P. abelii. All samples were successfully identified with high probabilities. The results obtained in this study contributes to improve the understanding of captive orangutans genetic identification and indirectly a guide to the authorities in developing plans for the management in captivity and conservation of primates in Malaysia using molecular data.
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Abstract
A large numerals of the orangutans genetic status in captivity is unknown to its exact thus complicates the translocation process to their true natural habitat. A study was carried out to identify 37 captive orangutans in Peninsular Malaysia at the species and subspecies level using mitochondrial DNA (mtDNA) displacement loop (D-loop) region DNA sequences. Orangutan genetic samples were provided by the Bukit Merah Orang Utan Island (BMOUI) Zoo Negara Zoo Taiping and the A Famosa. Total genomic DNA was extracted from both fecal and blood samples. Polymerase chain reaction (PCR) was performed using specific primers for the orang utan. Data analyses were carried out using distance and character based approaches namely the neighbor joining (NJ) maximum parsimony (MP) and Bayesian techniques. About 8 individuals were identified as Pongo pygmaeus pygmaeus 20 as P. p. morio 5 as P. p. wurmbii and 4 as P. abelii. All samples were successfully identified with high probabilities. The results obtained in this study contributes to improve the understanding of captive orangutans genetic identification and indirectly a guide to the authorities in developing plans for the management in captivity and conservation of primates in Malaysia using molecular data.
Additional Metadata
Item Type: | Article |
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AGROVOC Term: | Apes |
AGROVOC Term: | Primates |
AGROVOC Term: | Chordata |
AGROVOC Term: | Identification |
AGROVOC Term: | Genetics |
AGROVOC Term: | Mitochondrial genetics |
AGROVOC Term: | DNA |
AGROVOC Term: | DNA sequence |
AGROVOC Term: | genomics |
AGROVOC Term: | Polymerase chain reaction |
Depositing User: | Ms. Suzila Mohamad Kasim |
Last Modified: | 24 Apr 2025 06:29 |
URI: | http://webagris.upm.edu.my/id/eprint/25010 |
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