Short term, low temperature preservation of the pacific whiteleg shrimp Penaeus vannamei Spermatophores

Cataño, Yamilis and Intriago, Pablo and Shinn, Andrew P. and Prieto-Guevara, Martha and Atencio-García, Víctor (2025) Short term, low temperature preservation of the pacific whiteleg shrimp Penaeus vannamei Spermatophores. Asian Fisheries Science Journal (Malaysia), 38. pp. 1-7. ISSN 2073-3720

The study aimed to assess the efficacy of short-term (≤24 d), low-temperature preservation of Pacific whiteleg shrimp (Penaeus vannamei) spermatophores. A total of 432 spermatophores from 216 males, averaging 35.1 ± 3.1 g in weight, were preserved in mineral oil at temperatures of 10 °C and 24 °C. Sperm viability was monitored every 2 d over 24 d, evaluating fertility percentage, hatching rate, and nauplii deformity in females artificially inseminated with the preserved spermatophores compared with naturally inseminated females. In the control group, throughout the 24-d experiment, the average fertility, hatching rate, and nauplii count per gram of female were 66 ± 7 %, 94 ± 6 %, and 4,875 ± 148 nauplii g-1, respectively. Females inseminated with spermatophores stored at both temperatures exhibited a gradual decline in all measured parameters. Although spermatophores stored at 24 °C showed a linear decrease in fertility over time, this preservation technique proved to be effective for short-term storage of sperm, with good average hatching for up to 18 d at 10 °C (>50 %) and 8 d at 24 °C (>40 %). Notably, this technique has been successfully employed for the past seven years to facilitate controlled crossbreeding, mitigating the risk of pathogen contamination.

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Abstract

The study aimed to assess the efficacy of short-term (≤24 d), low-temperature preservation of Pacific whiteleg shrimp (Penaeus vannamei) spermatophores. A total of 432 spermatophores from 216 males, averaging 35.1 ± 3.1 g in weight, were preserved in mineral oil at temperatures of 10 °C and 24 °C. Sperm viability was monitored every 2 d over 24 d, evaluating fertility percentage, hatching rate, and nauplii deformity in females artificially inseminated with the preserved spermatophores compared with naturally inseminated females. In the control group, throughout the 24-d experiment, the average fertility, hatching rate, and nauplii count per gram of female were 66 ± 7 %, 94 ± 6 %, and 4,875 ± 148 nauplii g-1, respectively. Females inseminated with spermatophores stored at both temperatures exhibited a gradual decline in all measured parameters. Although spermatophores stored at 24 °C showed a linear decrease in fertility over time, this preservation technique proved to be effective for short-term storage of sperm, with good average hatching for up to 18 d at 10 °C (>50 %) and 8 d at 24 °C (>40 %). Notably, this technique has been successfully employed for the past seven years to facilitate controlled crossbreeding, mitigating the risk of pathogen contamination.

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Item Type:	Article
AGROVOC Term:	shrimp culture
AGROVOC Term:	preservation
AGROVOC Term:	storage
AGROVOC Term:	artificial insemination
AGROVOC Term:	mineral oils
AGROVOC Term:	Penaeus vannamei
AGROVOC Term:	fertility
Geographical Term:	Colombia
Depositing User:	Nor Hasnita Abdul Samat
Date Deposited:	09 Mar 2026 01:52
Last Modified:	09 Mar 2026 01:52
URI:	http://webagris.upm.edu.my/id/eprint/25058

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