In Vitro cultural studies for callogenesis and embryogenesis of oil palm (Elaeis guineensis Jacq.) using seedling and spear leaf explants

Kalyana Babu, B. and Mathur, R. K. and Suresh, K. and Ravichandran, G. and Susanthi, B. and Tiwari, S. (2025) In Vitro cultural studies for callogenesis and embryogenesis of oil palm (Elaeis guineensis Jacq.) using seedling and spear leaf explants. Journal of Oil Palm Research (Malaysia), 37 (1). pp. 64-71. ISSN 2811-4701

In oil palm, hybrids are not homogeneous due to their highly heterogeneous nature. However, the generation of homogeneous material is of prime importance, which can be possible only by using somatic embryogenesis using explants like spear leaf and seedling leaf. Evaluation of callogenesis and embryogenesis of oil palm using seedling and spear leaf is reported here. This is the first report on in vitro production of callus and somatic embryos using leaves as explants in India. Seedling leaf experiment revealed that callus induction ranged from 3%-4% among the four media combinations used in the study. Somatic embryo induction was observed in modified Murashige and Skoog (MS) containing picloram (37.26 μmol). The minimum time required for callus induction was 29 days whereas the maximum time required for initiation of callus was f ive months and four days. The minimum time required for induction of embryogenic callus was noted to be seven months and five days on two different media, whereas, the maximum time was 11 months and 14 days on modified N6 with dicamba (72.39 µmol) (palm No. 377). The present study opens up the prospect of understanding embryogenesis and manipulating in vitro culture conditions to achieve plantlet regeneration in oil palm using leaf explant.

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Abstract

In oil palm, hybrids are not homogeneous due to their highly heterogeneous nature. However, the generation of homogeneous material is of prime importance, which can be possible only by using somatic embryogenesis using explants like spear leaf and seedling leaf. Evaluation of callogenesis and embryogenesis of oil palm using seedling and spear leaf is reported here. This is the first report on in vitro production of callus and somatic embryos using leaves as explants in India. Seedling leaf experiment revealed that callus induction ranged from 3%-4% among the four media combinations used in the study. Somatic embryo induction was observed in modified Murashige and Skoog (MS) containing picloram (37.26 μmol). The minimum time required for callus induction was 29 days whereas the maximum time required for initiation of callus was f ive months and four days. The minimum time required for induction of embryogenic callus was noted to be seven months and five days on two different media, whereas, the maximum time was 11 months and 14 days on modified N6 with dicamba (72.39 µmol) (palm No. 377). The present study opens up the prospect of understanding embryogenesis and manipulating in vitro culture conditions to achieve plantlet regeneration in oil palm using leaf explant.

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Item Type:	Article
AGROVOC Term:	oil palms
AGROVOC Term:	callogenesis
AGROVOC Term:	somatic embryogenesis
AGROVOC Term:	in vitro culture
AGROVOC Term:	explants
AGROVOC Term:	somatic embryos
AGROVOC Term:	Elaeis guineensis
AGROVOC Term:	tissue culture
AGROVOC Term:	plant biotechnology
AGROVOC Term:	cell culture
Geographical Term:	India
Depositing User:	Nor Hasnita Abdul Samat
Date Deposited:	02 Apr 2026 07:26
Last Modified:	02 Apr 2026 07:26
URI:	http://webagris.upm.edu.my/id/eprint/25229

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