Analysis of putative EgBBM promoter binding proteins during oil palm somatic embryogenesis

Khianchaikhan, Kamolwan and Aroonluk, Suvichark and Vuttipongchaikij, Supachai and Jantasuriyarat, Chatchawan (2025) Analysis of putative EgBBM promoter binding proteins during oil palm somatic embryogenesis. Journal of Oil Palm Research (Malaysia), 37 (1). pp. 72-84. ISSN 2811-4701

BABY BOOM (BBM) gene has been identified as a crucial factor in regulating somatic embryogenesis in plants. During this process, high levels of BBM expression are observed. Nonetheless, the mechanisms regulating the expression of BBM during somatic embryogenesis in oil palm remain unclear. In this study, we cloned and examined the cis-regulatory elements of the EgBBM promoter. Our analysis revealed the presence of cis-regulatory elements, such as ABRE, as-1, G-box, Sp1, STRE, ARE, CAT-Box and MYB, which are similar to those found in the promoters of AtBBM and OsBBM1. In addition, we conducted a DNA-protein pull-down assay followed by mass spectrometry to identify the potential binding proteins of the EgBBM promoter. Several proteins were related to histone modification through direct and indirect interaction for controlling the EgBBM expression. Therefore, this study highlighted a complex regulatory network formation of histone modification and putative EgBBM binding proteins through both DNA-protein and protein-protein interactions. Our findings provide valuable insights into the factors that influence the role of EgBBM in oil palm tissue culture during somatic embryogenesis.

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Abstract

BABY BOOM (BBM) gene has been identified as a crucial factor in regulating somatic embryogenesis in plants. During this process, high levels of BBM expression are observed. Nonetheless, the mechanisms regulating the expression of BBM during somatic embryogenesis in oil palm remain unclear. In this study, we cloned and examined the cis-regulatory elements of the EgBBM promoter. Our analysis revealed the presence of cis-regulatory elements, such as ABRE, as-1, G-box, Sp1, STRE, ARE, CAT-Box and MYB, which are similar to those found in the promoters of AtBBM and OsBBM1. In addition, we conducted a DNA-protein pull-down assay followed by mass spectrometry to identify the potential binding proteins of the EgBBM promoter. Several proteins were related to histone modification through direct and indirect interaction for controlling the EgBBM expression. Therefore, this study highlighted a complex regulatory network formation of histone modification and putative EgBBM binding proteins through both DNA-protein and protein-protein interactions. Our findings provide valuable insights into the factors that influence the role of EgBBM in oil palm tissue culture during somatic embryogenesis.

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Item Type:	Article
AGROVOC Term:	oil palms
AGROVOC Term:	somatic embryogenesis
AGROVOC Term:	gene expression
AGROVOC Term:	tissue culture
AGROVOC Term:	promoters
AGROVOC Term:	binding proteins
AGROVOC Term:	gene regulatory networks
AGROVOC Term:	Mimosa scabrella
AGROVOC Term:	mass spectrometry
Geographical Term:	Thailand
Depositing User:	Nor Hasnita Abdul Samat
Date Deposited:	18 Jun 2025 04:22
Last Modified:	02 Apr 2026 07:53
URI:	http://webagris.upm.edu.my/id/eprint/25230

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