Yeast extract increases biomass, antioxidant, anti-inflammatory, and anticancer of Cichorium intybus L. hairy roots

Mai, N. T. P. and Hilbert, J. L. (2025) Yeast extract increases biomass, antioxidant, anti-inflammatory, and anticancer of Cichorium intybus L. hairy roots. International Food Research Journal (Malaysia), 32 (2). pp. 465-474. ISSN 2231 7546

Cichorium intybus L. hairy roots (HRs), induced from chicory plants by Rhizobium rhizogenes, have demonstrated potential antioxidant and antimicrobial activities. These chicory HRs exhibit bioactivities due to the presence of chemical compounds such as polyphenols and flavonoids. In the present work, yeast extract (YE) was evaluated as a biostimulant for HRs. After 25 days of culturing HRs in a medium containing YE at two different concentrations, the sample cultivated with 50 mg/L of YE showed higher biomass accumulation than the one treated with 100 mg/L. Specifically, 50 mg/L of YE increased HR biomass approximately 2.5 times compared to the control. Additionally, YE enhanced antioxidant enzyme activities (catalase and peroxidase) by increasing phenolic and flavonoid contents. The anti-inflammatory activity was assessed using the nitric oxide (NO) inhibition test, revealing that YE-elicited HRs suppressed NO synthesis at a nontoxic concentration of 5 μg/mL. Furthermore, YE-elicited HRs inhibited the proliferation of HepG2 cancer cells by approximately 20%. Overall, the present work demonstrated that YE effectively stimulated HR biomass growth while enhancing antioxidant, antiinflammatory, and anticancer activities, providing a promising strategy for cultivating C. intybus L. hairy roots for therapeutic applications.

Full text available from: Official URL: http://www.ifrj.upm.edu.my/32%20(02)%202025/11%20-...

Abstract

Cichorium intybus L. hairy roots (HRs), induced from chicory plants by Rhizobium rhizogenes, have demonstrated potential antioxidant and antimicrobial activities. These chicory HRs exhibit bioactivities due to the presence of chemical compounds such as polyphenols and flavonoids. In the present work, yeast extract (YE) was evaluated as a biostimulant for HRs. After 25 days of culturing HRs in a medium containing YE at two different concentrations, the sample cultivated with 50 mg/L of YE showed higher biomass accumulation than the one treated with 100 mg/L. Specifically, 50 mg/L of YE increased HR biomass approximately 2.5 times compared to the control. Additionally, YE enhanced antioxidant enzyme activities (catalase and peroxidase) by increasing phenolic and flavonoid contents. The anti-inflammatory activity was assessed using the nitric oxide (NO) inhibition test, revealing that YE-elicited HRs suppressed NO synthesis at a nontoxic concentration of 5 μg/mL. Furthermore, YE-elicited HRs inhibited the proliferation of HepG2 cancer cells by approximately 20%. Overall, the present work demonstrated that YE effectively stimulated HR biomass growth while enhancing antioxidant, antiinflammatory, and anticancer activities, providing a promising strategy for cultivating C. intybus L. hairy roots for therapeutic applications.

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Item Type:	Article
AGROVOC Term:	chicory
AGROVOC Term:	biomass production
AGROVOC Term:	cell culture
AGROVOC Term:	yeast extracts
AGROVOC Term:	biostimulants
AGROVOC Term:	biomass
AGROVOC Term:	polyphenols
AGROVOC Term:	flavonoids
AGROVOC Term:	nitric oxide
Geographical Term:	Vietnam
Depositing User:	Nor Hasnita Abdul Samat
Date Deposited:	26 May 2026 07:18
Last Modified:	26 May 2026 07:18
URI:	http://webagris.upm.edu.my/id/eprint/25328

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