Impact of infusion time and ultrasound-assisted extraction on bioactive compounds and antioxidant capacity of foods prepared from seeds and leaves of Ramon tree (Brosimum alicastrum)

Gullian Klanian, M. and Sánchez Solís, M. J. (2024) Impact of infusion time and ultrasound-assisted extraction on bioactive compounds and antioxidant capacity of foods prepared from seeds and leaves of Ramon tree (Brosimum alicastrum). International Food Research Journal (Malaysia), 31. pp. 588-600. ISSN 2231 7546

The objective of the present work was to analyse the concentrations of bioactive compounds, and the antioxidant capacity of three foods prepared from the seeds and leaves of Ramon tree (Brosimum alicastrum). We studied the effect of infusion time and ultrasonic extraction on leaves, roasted seeds (coffee substitute), and Ramon flour to optimise the recovery of bioactive components. Infusion time positively affected the concentrations of total phenolic content (TPC), total flavonoid content (TFC), total tannins (TCT), and glycosylated flavonoids (TFG) in the leaves and seeds. The amount of solubilised phenolic compounds increased with increasing infusion time until 2,552 mg of TPC, 54.1 mg of TFC, 75.3 mg of TFG, and 2.40 mg of TCT were obtained per 100 g of leaves. We reported 32.9% greater infusion of toasted seeds in TPC, 87.3% in anthocyanins, 84.6% in TCT, and 91.5% in vitamin C than in leaves. Furthermore, the antioxidant capacity of the seed infusions was 56% greater than that of the leaf infusions. The ultrasonication time was positively correlated with the tannin concentration, which was 43.8% greater in the leaves (5.24 mg/100 g) than in the flour. The neutralising capacity of free radicals determined by 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) was 23.6% greater than that of flour (27.93 µmol/g) and leaves (6.48 µmol/g), and was not related to the extraction time. Taken together, these findings showed that Ramon seeds and leaves could be highly functional foods due to their nutritional quality and high content of antioxidant compounds.

Full text available from: Official URL: http://www.ifrj.upm.edu.my/31%20(03)%202024/05%20-...

Abstract

The objective of the present work was to analyse the concentrations of bioactive compounds, and the antioxidant capacity of three foods prepared from the seeds and leaves of Ramon tree (Brosimum alicastrum). We studied the effect of infusion time and ultrasonic extraction on leaves, roasted seeds (coffee substitute), and Ramon flour to optimise the recovery of bioactive components. Infusion time positively affected the concentrations of total phenolic content (TPC), total flavonoid content (TFC), total tannins (TCT), and glycosylated flavonoids (TFG) in the leaves and seeds. The amount of solubilised phenolic compounds increased with increasing infusion time until 2,552 mg of TPC, 54.1 mg of TFC, 75.3 mg of TFG, and 2.40 mg of TCT were obtained per 100 g of leaves. We reported 32.9% greater infusion of toasted seeds in TPC, 87.3% in anthocyanins, 84.6% in TCT, and 91.5% in vitamin C than in leaves. Furthermore, the antioxidant capacity of the seed infusions was 56% greater than that of the leaf infusions. The ultrasonication time was positively correlated with the tannin concentration, which was 43.8% greater in the leaves (5.24 mg/100 g) than in the flour. The neutralising capacity of free radicals determined by 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) was 23.6% greater than that of flour (27.93 µmol/g) and leaves (6.48 µmol/g), and was not related to the extraction time. Taken together, these findings showed that Ramon seeds and leaves could be highly functional foods due to their nutritional quality and high content of antioxidant compounds.

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Item Type:	Article
AGROVOC Term:	seeds
AGROVOC Term:	leaves
AGROVOC Term:	flours
AGROVOC Term:	phenolic compounds
AGROVOC Term:	flavonoids
AGROVOC Term:	tannins
AGROVOC Term:	antioxidants
AGROVOC Term:	functional foods
AGROVOC Term:	coffee substitutes
AGROVOC Term:	ascorbic acid
Geographical Term:	Mexico
Uncontrolled Keywords:	Brosimum alicastrum, Ramon, Mayan nut, polyphenols, antioxidant activity
Depositing User:	Nor Hasnita Abdul Samat
Date Deposited:	23 Feb 2026 04:11
Last Modified:	23 Feb 2026 04:11
URI:	http://webagris.upm.edu.my/id/eprint/2616

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