Effect of polyphenols from kiwi by-products (PKWP) on redox and metabolic homeostasis of HepG2 cells

Wang, J. and Jin, D. L. and Fang, L. L. and Yu, J. F. and Wang, M. and Yang, W. J. and Yao, W. B. and Wang, J. K. and Li, N. and Gong, P. (2024) Effect of polyphenols from kiwi by-products (PKWP) on redox and metabolic homeostasis of HepG2 cells. International Food Research Journal (Malaysia), 31. pp. 770-783. ISSN 2231 7546

Polyphenols from kiwi by-products (PKWP) have been previously reported to have an inhibitory effect on cancer cells; but, the potential anti-cancer mechanism remains unknown. Metabolic alterations in cancer cells provide bioenergy and substances for uncontrolled proliferation and development, and interfering with the metabolic pathways has been regarded as effective in impeding cancer progression. Cancer cells are also characterised by a relatively higher level of oxidative stress and an enhanced antioxidant defence system, facilitating multiple stages of tumorigenesis. Anti-cancer mechanisms of PKWP based on metabolic and redox homeostasis in HepG2 cells were thus investigated in the present work. The results showed that PKWP effectively decreased HepG2 cell viability in a concentration-dependent manner. PKWP caused metabolic disorders in HepG2 cells, and significantly affected the content of about 32 metabolites. PKWP mainly inhibited saccharide synthesis and glycolysis, and restricted the utilisation of amino acids. PKWP stimulated ROS production, and caused lipid peroxidation, resulting in oxidative damage. PKWP also down-regulated the activities of T-SOD and CAT, and the level of GSH, and inhibited the protein expressions of HO-1 and COX-2, weakening the cellular antioxidant capacity. Thus, PKWP could exert prominent anti-cancer activity in HepG2 cells by disrupting metabolic homeostasis, and induction of oxidative stress. These findings will provide evidence for further elucidation of the anti-cancer mechanism of PKWP, and the potential application of PKWP as a natural ingredient in functional food, cosmetic, and pharmaceutical industries.

Full text available from: Official URL: http://www.ifrj.upm.edu.my/31%20(03)%202024/20%20-...

Abstract

Polyphenols from kiwi by-products (PKWP) have been previously reported to have an inhibitory effect on cancer cells; but, the potential anti-cancer mechanism remains unknown. Metabolic alterations in cancer cells provide bioenergy and substances for uncontrolled proliferation and development, and interfering with the metabolic pathways has been regarded as effective in impeding cancer progression. Cancer cells are also characterised by a relatively higher level of oxidative stress and an enhanced antioxidant defence system, facilitating multiple stages of tumorigenesis. Anti-cancer mechanisms of PKWP based on metabolic and redox homeostasis in HepG2 cells were thus investigated in the present work. The results showed that PKWP effectively decreased HepG2 cell viability in a concentration-dependent manner. PKWP caused metabolic disorders in HepG2 cells, and significantly affected the content of about 32 metabolites. PKWP mainly inhibited saccharide synthesis and glycolysis, and restricted the utilisation of amino acids. PKWP stimulated ROS production, and caused lipid peroxidation, resulting in oxidative damage. PKWP also down-regulated the activities of T-SOD and CAT, and the level of GSH, and inhibited the protein expressions of HO-1 and COX-2, weakening the cellular antioxidant capacity. Thus, PKWP could exert prominent anti-cancer activity in HepG2 cells by disrupting metabolic homeostasis, and induction of oxidative stress. These findings will provide evidence for further elucidation of the anti-cancer mechanism of PKWP, and the potential application of PKWP as a natural ingredient in functional food, cosmetic, and pharmaceutical industries.

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Item Type:	Article
AGROVOC Term:	Actinidia
AGROVOC Term:	kiwifruits
AGROVOC Term:	fruit peels
AGROVOC Term:	polyphenols
AGROVOC Term:	amino acids
AGROVOC Term:	functional foods
AGROVOC Term:	oxidative stress
AGROVOC Term:	glycolysis
AGROVOC Term:	lipid peroxidation
AGROVOC Term:	medicinal properties
Geographical Term:	China
Uncontrolled Keywords:	anti-cancer activity, metabolism, oxidative stress, PKWP, polyphenols
Depositing User:	Nor Hasnita Abdul Samat
Date Deposited:	23 Feb 2026 04:46
Last Modified:	23 Feb 2026 04:46
URI:	http://webagris.upm.edu.my/id/eprint/2644

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