Sterilisation Protocols and in vitro Shoot and Root Induction of Zingiber officinale var. Rosc. using 6-Benzylaminopurine (BAP) and Naphthalene Acetic Acid (NAA)

Nurul Izzah Syafiqah Salim, . and Siti Nurul Atikah Abu Samah, . and Shamsiah Abdullah, . and Nor Azma Yusuf, . (2024) Sterilisation Protocols and in vitro Shoot and Root Induction of Zingiber officinale var. Rosc. using 6-Benzylaminopurine (BAP) and Naphthalene Acetic Acid (NAA). Journal of Agrobiotechnology (Malaysia), 15. pp. 1-10. ISSN 2180-1983

Bentong ginger is the most well-known variety of Zingiber officinale in Malaysia, owing to its extensive use in traditional medicine and as a culinary spice. It is vegetatively propagated, and large quantities of rhizomes are required as initial planting materials. In addition, a variety of different soil-borne diseases are associated with the vegetative propagation of ginger using its rhizomes; hence, producing them on a massive scale requires a lot of time. Plant tissue culture techniques have utilised many plant species to produce disease-free planting materials. Therefore, the study aimed to determine the effects of ethanol on the surface sterilisation of shoot bud explants as well as the optimisation of 6-Benzylaminopurine (BAP) and Naphthalene acetic acid (NAA) on shoot and root propagation. The shoot bud explant was successfully sterilised using 20% (v/v) of Clorox and Tween20 for 30 minutes and 95% (v/v) of ethanol for 1 minute, resulting in a 58% contamination-free explant. The highest number of shoots and roots were initiated from shoot bud explants of Z. officinale on MS solid medium supplemented with 4.0 mg/l BAP and 1.5 mg/L NAA, respectively. MS medium incorporated with a combination of 3.0 mg/l BAP and 1.5 mg/l NAA produced the highest length of shoot. These results suggested that the protocols used can be adopted for large-scale propagation of disease-free planting materials of Zingiber officinale var Rosc.

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Abstract

Bentong ginger is the most well-known variety of Zingiber officinale in Malaysia, owing to its extensive use in traditional medicine and as a culinary spice. It is vegetatively propagated, and large quantities of rhizomes are required as initial planting materials. In addition, a variety of different soil-borne diseases are associated with the vegetative propagation of ginger using its rhizomes; hence, producing them on a massive scale requires a lot of time. Plant tissue culture techniques have utilised many plant species to produce disease-free planting materials. Therefore, the study aimed to determine the effects of ethanol on the surface sterilisation of shoot bud explants as well as the optimisation of 6-Benzylaminopurine (BAP) and Naphthalene acetic acid (NAA) on shoot and root propagation. The shoot bud explant was successfully sterilised using 20% (v/v) of Clorox and Tween20 for 30 minutes and 95% (v/v) of ethanol for 1 minute, resulting in a 58% contamination-free explant. The highest number of shoots and roots were initiated from shoot bud explants of Z. officinale on MS solid medium supplemented with 4.0 mg/l BAP and 1.5 mg/L NAA, respectively. MS medium incorporated with a combination of 3.0 mg/l BAP and 1.5 mg/l NAA produced the highest length of shoot. These results suggested that the protocols used can be adopted for large-scale propagation of disease-free planting materials of Zingiber officinale var Rosc.

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Item Type:	Article
AGROVOC Term:	ginger
AGROVOC Term:	culinary herbs
AGROVOC Term:	Zingiber officinale
AGROVOC Term:	sterilization
AGROVOC Term:	propagation materials
AGROVOC Term:	tissue culture
AGROVOC Term:	6-benzylaminopurine
AGROVOC Term:	ethanol
AGROVOC Term:	plant growth
AGROVOC Term:	growth rate
Geographical Term:	Malaysia
Uncontrolled Keywords:	Micropropagation, tissue culture, halia bentong, Zingiber officinale, Zingiberaceae
Depositing User:	Nor Hasnita Abdul Samat
Date Deposited:	19 Mar 2026 02:05
Last Modified:	19 Mar 2026 02:05
URI:	http://webagris.upm.edu.my/id/eprint/2663

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