Initial characterisation of Metarhizium anisopliae CPMa1502 for the development of a biopesticide against the oil palm fruit scraper Demotispa neivai (Coleoptera: Chrysomelidae)

Quiroga-Cubides, Ginna and Borrero-Echeverry, Felipe and María Jiménez, Ana and Montes-Bazurto, Luis Guillermo and Pardey, Alex Bustillo and Gómez, Martha I and Cuartas-Otálora, Paola E (2024) Initial characterisation of Metarhizium anisopliae CPMa1502 for the development of a biopesticide against the oil palm fruit scraper Demotispa neivai (Coleoptera: Chrysomelidae). Journal of Oil Palm Research (Malaysia), 36. pp. 40-50. ISSN 2811-4701

Entomopathogenic fungi are key components of biological pest control programs. Among these, Metarhizium anisopliae is one of the best-studied and has been demonstrated to be particularly effective against coleopterans. The native M. anisopliae CPMa1502, previously selected for its insecticidal activity against Demotispa neivai (oil palm fruit scraper), was studied to establish its growth parameters under several culture conditions and its virulence on different stages of D. neivai. Considerable conidia production of CPMa1502 on MAYP (Maltose Agar Yeast with Potato Extract) and SMAYR (Sabouraud Maltose Agar with yeast extract and rice extract) agar was observed (1x109 conidia cm–2 at 14 days). The isolate was highly tolerant to a wide range of pH (5-9; germination >90%; radial growth rate: 0.95-1.02 mm day–1) but moderately tolerant to high temperatures (>35°C). Additionally, the adhesion capacity to D. neivai cuticle and conidial hydrophobicity was strong (114 conidia mm–2: 72%). Finally, the insecticidal activity of CPMa1502 was greater on larvae than on adults, with a mean lethal concentration (LC50 ) of 1.8x104 conidia mL–1 and a mean lethal time (LT50 ) of 4.3 days, than on adults. These findings show that CPMa1502 is a promising candidate for further development as a biopesticide against D. neivai.

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Abstract

Entomopathogenic fungi are key components of biological pest control programs. Among these, Metarhizium anisopliae is one of the best-studied and has been demonstrated to be particularly effective against coleopterans. The native M. anisopliae CPMa1502, previously selected for its insecticidal activity against Demotispa neivai (oil palm fruit scraper), was studied to establish its growth parameters under several culture conditions and its virulence on different stages of D. neivai. Considerable conidia production of CPMa1502 on MAYP (Maltose Agar Yeast with Potato Extract) and SMAYR (Sabouraud Maltose Agar with yeast extract and rice extract) agar was observed (1x109 conidia cm–2 at 14 days). The isolate was highly tolerant to a wide range of pH (5-9; germination >90%; radial growth rate: 0.95-1.02 mm day–1) but moderately tolerant to high temperatures (>35°C). Additionally, the adhesion capacity to D. neivai cuticle and conidial hydrophobicity was strong (114 conidia mm–2: 72%). Finally, the insecticidal activity of CPMa1502 was greater on larvae than on adults, with a mean lethal concentration (LC50 ) of 1.8x104 conidia mL–1 and a mean lethal time (LT50 ) of 4.3 days, than on adults. These findings show that CPMa1502 is a promising candidate for further development as a biopesticide against D. neivai.

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Item Type:	Article
AGROVOC Term:	oil palms
AGROVOC Term:	pest control
AGROVOC Term:	biological control
AGROVOC Term:	Metarhizium anisopliae
AGROVOC Term:	Fungi
AGROVOC Term:	hydrophobicity
AGROVOC Term:	virulence
AGROVOC Term:	larvae
Geographical Term:	Colombia
Uncontrolled Keywords:	biocontrol activity, conidia production, Elaeis guineensis, fruit scraper, growth parameters
Depositing User:	Nor Hasnita Abdul Samat
Date Deposited:	19 Mar 2026 02:09
Last Modified:	19 Mar 2026 02:09
URI:	http://webagris.upm.edu.my/id/eprint/2671

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