Effects of culture medium and light durations on the fungal growth of Bipolaris sorokiniana

Maizan Ismail, . and Abdul Shukor Juraimi, . and Abu Seman Idris, . and Mohd Hefni Rusli, . and Raja Zulkifli Raja Omar, . and Kamil A. Tohiran, . and Mohd Naqiuddin Husri, . (2024) Effects of culture medium and light durations on the fungal growth of Bipolaris sorokiniana. Journal of Oil Palm Research (Malaysia), 36. pp. 201-209. ISSN 2811-4701

Bipolaris sorokiniana has been identified as a potential indigenous fungal plant pathogen to control goosegrass (Eleusine indica) in oil palm plantations. However, for mass production, a suitable medium and an optimum growing environment need to be identified. This study was conducted to determine the suitable culture media and light duration requirements for optimal growth of B. sorokiniana. Four types of culture media: Potato dextrose agar (PDA), corn meal agar (CMA), V8 agar (V8A) and water agar (WA), were evaluated for the B. sorokiniana subculture. Three light durations (0, 12 and 24 hr) were tested for B. sorokiniana growth. The growth diameter and colony-forming units of B. sorokiniana were recorded. When compared to CMA, V8A and WA media in the same light durations, B. sorokiniana grew at maximum mean growth diameter at 12 hr light duration in PDA (4.56 cm), 24 hr light duration in PDA (4.36 cm) and 0 hr light duration in PDA (4.21 cm). Bipolaris sorokiniana produced more CFU during the 0 light duration than during the 12 hr and 24 hr light durations. Hence, this study shows that B. sorokiniana has the potential for reproduction using PDA culture media without light.

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Abstract

Bipolaris sorokiniana has been identified as a potential indigenous fungal plant pathogen to control goosegrass (Eleusine indica) in oil palm plantations. However, for mass production, a suitable medium and an optimum growing environment need to be identified. This study was conducted to determine the suitable culture media and light duration requirements for optimal growth of B. sorokiniana. Four types of culture media: Potato dextrose agar (PDA), corn meal agar (CMA), V8 agar (V8A) and water agar (WA), were evaluated for the B. sorokiniana subculture. Three light durations (0, 12 and 24 hr) were tested for B. sorokiniana growth. The growth diameter and colony-forming units of B. sorokiniana were recorded. When compared to CMA, V8A and WA media in the same light durations, B. sorokiniana grew at maximum mean growth diameter at 12 hr light duration in PDA (4.56 cm), 24 hr light duration in PDA (4.36 cm) and 0 hr light duration in PDA (4.21 cm). Bipolaris sorokiniana produced more CFU during the 0 light duration than during the 12 hr and 24 hr light durations. Hence, this study shows that B. sorokiniana has the potential for reproduction using PDA culture media without light.

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Item Type:	Article
AGROVOC Term:	Fungi
AGROVOC Term:	oil palms
AGROVOC Term:	Eleusine indica
AGROVOC Term:	biomass production
AGROVOC Term:	Bipolaris sorokiniana
AGROVOC Term:	culture media
AGROVOC Term:	potato dextrose agar
Geographical Term:	Malaysia
Depositing User:	Nor Hasnita Abdul Samat
Date Deposited:	18 Mar 2026 06:58
Last Modified:	18 Mar 2026 06:58
URI:	http://webagris.upm.edu.my/id/eprint/2702

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