Phenolics-enhancing Piper sarmentosum (Roxburgh) extracts pre-treated with supercritical carbon dioxide and its correlation with cytotoxicity and α-glucosidase inhibitory activities

Mohd Shahrul Ridzuan Hamil, . and Fauziahanim Zakaria, . and Lee, Chong Yew and Norzilawati Pauzi, . and Khamsah Suryati Mohd, . and Noor Hafizoh Saidan, . (2024) Phenolics-enhancing Piper sarmentosum (Roxburgh) extracts pre-treated with supercritical carbon dioxide and its correlation with cytotoxicity and α-glucosidase inhibitory activities. Pertanika Journal of Science & Technology (Malaysia), 32 (1). 323 -337. ISSN 2231-8526

Piper sarmentosum was found to have various phytochemicals such as polyphenols and flavonoids but also contains asarone isomers, which were reported as carcinogenic in several preclinical studies. Therefore, reducing asarone isomers from this functional food is important while retaining its functional properties. This study compares the total phenolics, total flavonoids, vitexin and naringenin contents, cytotoxicity, and anti-hyperglycaemic activity between various solvent extracts using a two-step extraction with SC-CO₂ pre-treatment and a single-step conventional solvent extraction without SC-CO₂ pre-treatment. The findings showed that phenolic content was significantly enhanced in ethanol, 50% ethanol, and water extracts pre-treated with SC-CO₂, significantly correlated with α-glucosidase inhibitory activity. SC-CO₂ pre-treated extracts enhanced the viability of two normal fibroblasts NIH/3T3 and CCD-18Co cell lines. It is concluded that SC-CO₂ extraction offers a rapid pre-treatment step to produce safer extracts with better quality and efficacy.

Full text available from: Official URL: http://www.pertanika.upm.edu.my/resources/files/Pe...

Abstract

Piper sarmentosum was found to have various phytochemicals such as polyphenols and flavonoids but also contains asarone isomers, which were reported as carcinogenic in several preclinical studies. Therefore, reducing asarone isomers from this functional food is important while retaining its functional properties. This study compares the total phenolics, total flavonoids, vitexin and naringenin contents, cytotoxicity, and anti-hyperglycaemic activity between various solvent extracts using a two-step extraction with SC-CO₂ pre-treatment and a single-step conventional solvent extraction without SC-CO₂ pre-treatment. The findings showed that phenolic content was significantly enhanced in ethanol, 50% ethanol, and water extracts pre-treated with SC-CO₂, significantly correlated with α-glucosidase inhibitory activity. SC-CO₂ pre-treated extracts enhanced the viability of two normal fibroblasts NIH/3T3 and CCD-18Co cell lines. It is concluded that SC-CO₂ extraction offers a rapid pre-treatment step to produce safer extracts with better quality and efficacy.

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Item Type:	Article
AGROVOC Term:	climbers
AGROVOC Term:	betel
AGROVOC Term:	polyphenols
AGROVOC Term:	flavonoids
AGROVOC Term:	plant extracts
AGROVOC Term:	chemical analysis
AGROVOC Term:	quality
AGROVOC Term:	inhibition
AGROVOC Term:	cytotoxicity
Geographical Term:	Malaysia
Uncontrolled Keywords:	supercritical fluid extraction, α-glucosidase
Depositing User:	Ms. Azariah Hashim
Date Deposited:	24 Nov 2025 03:19
Last Modified:	24 Nov 2025 03:19
URI:	http://webagris.upm.edu.my/id/eprint/2770

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