Citation
Nurul Nadhirah, . and Haja Maideen, . and Ab Rahman Zuraida, . and Othman Ayu Nazreena, . (2024) Spore germination of Diplazium simplicivenium Holtt. (Athyriaceae) in Peninsular Malaysia. Pertanika Journal Tropical Agricultural Science (Malaysia), 47 (2). pp. 411-419. ISSN 1511-3701
Abstract
Diplazium simplicivenium Holtt. a species of fern in Peninsular Malaysia. This study reports an efficient method for D. simplicivenium spore sterilisation and the effect of plant growth regulators (PGRs) via green globular bodies (GGB). Sterilisation with 0.1% mercuric chloride effectively allowed spore germination. The result showed that the culture media supplemented with 0.5 mg/L gibberellin positively affects the weight of GGB and the number of shoots with significant differences (ANOVA, p < 0.05). The soaking technique established in this study for spore sterilisation is an efficient approach, and the optimal plant and concentration of plant hormones were identified. This procedure can be applied to other indigenous ferns and closely similar species.
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Abstract
Diplazium simplicivenium Holtt. a species of fern in Peninsular Malaysia. This study reports an efficient method for D. simplicivenium spore sterilisation and the effect of plant growth regulators (PGRs) via green globular bodies (GGB). Sterilisation with 0.1% mercuric chloride effectively allowed spore germination. The result showed that the culture media supplemented with 0.5 mg/L gibberellin positively affects the weight of GGB and the number of shoots with significant differences (ANOVA, p < 0.05). The soaking technique established in this study for spore sterilisation is an efficient approach, and the optimal plant and concentration of plant hormones were identified. This procedure can be applied to other indigenous ferns and closely similar species.
Additional Metadata
| Item Type: | Article |
|---|---|
| AGROVOC Term: | ferns |
| AGROVOC Term: | spores |
| AGROVOC Term: | spore germination |
| AGROVOC Term: | sterilization |
| AGROVOC Term: | cultured meat |
| AGROVOC Term: | gibberellins |
| AGROVOC Term: | mercuric chloride |
| AGROVOC Term: | hormones |
| AGROVOC Term: | plant propagation |
| Geographical Term: | Malaysia |
| Uncontrolled Keywords: | Diplazium, fern, in vitro, micropropagation, spore |
| Depositing User: | Ms. Azariah Hashim |
| Date Deposited: | 19 Mar 2026 02:26 |
| Last Modified: | 19 Mar 2026 02:26 |
| URI: | http://webagris.upm.edu.my/id/eprint/2907 |
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