Indonesian native chicken eggshell membranes as source for collagen: optimum conditions and general characteristics

Febrisiantosa, A. and Wahyono, T. and Pratiwi, D. and Ujilestari, T. and Permadi, S. N. and Fajri, M. and Hakim, L. and Wahyuningsih, R. (2024) Indonesian native chicken eggshell membranes as source for collagen: optimum conditions and general characteristics. International Food Research Journal (Malaysia), 31. pp. 822-834. ISSN 2231 7546

High quantity of Indonesian native chicken eggshell membrane is available as a byproduct of the hatchery industry. The eggshell has a membrane-like interior, and still contains approximately 10% of the membrane’s total protein. In the present work, membranes from Indonesian chicken eggs sourced from a hatchery could have the potential to be a source of collagen. Based on the interaction of two variables (enzyme concentration and hydrolysis duration), response surface methodology (RSM) with a central composite design was utilised to identify the optimum collagen extraction conditions. Using 5 M acetic acid and pepsin (85, 90, and 95 U/mg defatted skin) at 4°C for 72, 96, and 120 h, the collagen was extracted. The optimal conditions yielded 31% collagen with a hydrolysis duration of 120 h, and an enzyme concentration of 90 U/mg. The optimal yield was characterised using colour analysis, soluble protein, and FTIR. Collagen from the Indonesian native chicken eggshell membrane showed similar properties with standard collagen sources.

Full text available from: Official URL: http://www.ifrj.upm.edu.my/31%20(04)%202024/02%20-...

Abstract

High quantity of Indonesian native chicken eggshell membrane is available as a byproduct of the hatchery industry. The eggshell has a membrane-like interior, and still contains approximately 10% of the membrane’s total protein. In the present work, membranes from Indonesian chicken eggs sourced from a hatchery could have the potential to be a source of collagen. Based on the interaction of two variables (enzyme concentration and hydrolysis duration), response surface methodology (RSM) with a central composite design was utilised to identify the optimum collagen extraction conditions. Using 5 M acetic acid and pepsin (85, 90, and 95 U/mg defatted skin) at 4°C for 72, 96, and 120 h, the collagen was extracted. The optimal conditions yielded 31% collagen with a hydrolysis duration of 120 h, and an enzyme concentration of 90 U/mg. The optimal yield was characterised using colour analysis, soluble protein, and FTIR. Collagen from the Indonesian native chicken eggshell membrane showed similar properties with standard collagen sources.

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Item Type:	Article
AGROVOC Term:	collagen
AGROVOC Term:	sugar-beet byproducts
AGROVOC Term:	extraction
AGROVOC Term:	hydrolysis
AGROVOC Term:	characterization
AGROVOC Term:	enzymes
AGROVOC Term:	pepsin
AGROVOC Term:	acetic acid
Geographical Term:	Indonesian
Depositing User:	Nor Hasnita Abdul Samat
Date Deposited:	18 Mar 2026 02:31
Last Modified:	18 Mar 2026 02:31
URI:	http://webagris.upm.edu.my/id/eprint/3073

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