Prevalence of Vibrio parahaemolyticus, Vibrio cholerae, and Vibrio alginolyticus in a white-leg shrimp (Litopenaeus vannamei) farm in Sarawak

Dalene Lesen, . and Elexson Nillian, . and Dayang Najwa Awang Baki, . and Tunung Robin, . (2024) Prevalence of Vibrio parahaemolyticus, Vibrio cholerae, and Vibrio alginolyticus in a white-leg shrimp (Litopenaeus vannamei) farm in Sarawak. Pertanika Journal of Science & Technology (Malaysia), 32. pp. 2233-2257. ISSN 2231-8526

Vibriosis in shrimp farms poses potential risks to the industry’s sustainability and the consumers’ health. Little is known about the dynamics and variation of Vibrio spp—population in shrimp production in Sarawak, Malaysia. The apparent prevalence of three Vibrio species, V. parahaemolyticus, V. cholerae, and V. alginolyticus, were investigated in water (n=12), sediment (n=12), shrimp (n=12), and effluent (n=12) samples collected from two ponds in a coastal shrimp farm during one production cycle. Multiplex-PCR using specific primer sets showed the presence of the three Vibrio species. Based on the results, V. parahaemolyticus was the most prevalent in all four samples, with a contamination rate of 97.92% (95% CI: 89. 10 to 99.89%), while V. cholerae and V. alginolyticus had a contamination rate of 47.92% (95% CI: 34.37 to 61.67%) and 25.0% (95% CI: 14.92 to 38.78%), respectively. High Vibrio load in the shrimp farm is due to favourable environmental factors, such as optimal temperatures, salinity, and pH ranges for the growth of these species. The study’s findings offer important preliminary insights into the prevalence and distribution of these pathogenic Vibrio spp., within a shrimp farm in Kuching, Sarawak. This study serves as a potential model for monitoring Vibrio spp. prevalence in other shrimp farms across Sarawak, thereby addressing the scarcity of data on prevalence in the region.

Full text available from: Official URL: http://www.pertanika.upm.edu.my/resources/files/Pe...

Abstract

Vibriosis in shrimp farms poses potential risks to the industry’s sustainability and the consumers’ health. Little is known about the dynamics and variation of Vibrio spp—population in shrimp production in Sarawak, Malaysia. The apparent prevalence of three Vibrio species, V. parahaemolyticus, V. cholerae, and V. alginolyticus, were investigated in water (n=12), sediment (n=12), shrimp (n=12), and effluent (n=12) samples collected from two ponds in a coastal shrimp farm during one production cycle. Multiplex-PCR using specific primer sets showed the presence of the three Vibrio species. Based on the results, V. parahaemolyticus was the most prevalent in all four samples, with a contamination rate of 97.92% (95% CI: 89. 10 to 99.89%), while V. cholerae and V. alginolyticus had a contamination rate of 47.92% (95% CI: 34.37 to 61.67%) and 25.0% (95% CI: 14.92 to 38.78%), respectively. High Vibrio load in the shrimp farm is due to favourable environmental factors, such as optimal temperatures, salinity, and pH ranges for the growth of these species. The study’s findings offer important preliminary insights into the prevalence and distribution of these pathogenic Vibrio spp., within a shrimp farm in Kuching, Sarawak. This study serves as a potential model for monitoring Vibrio spp. prevalence in other shrimp farms across Sarawak, thereby addressing the scarcity of data on prevalence in the region.

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Item Type:	Article
AGROVOC Term:	aquaculture
AGROVOC Term:	seafood safety
AGROVOC Term:	shrimp culture
AGROVOC Term:	sampling
AGROVOC Term:	statistical methods
AGROVOC Term:	Vibrio parahaemolyticus
AGROVOC Term:	health hazards
AGROVOC Term:	food safety
Geographical Term:	Malaysia
Depositing User:	Ms. Azariah Hashim
Date Deposited:	06 Feb 2026 01:48
Last Modified:	06 Feb 2026 01:48
URI:	http://webagris.upm.edu.my/id/eprint/3166

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