Isolation and characterisation of two lytic bacteriophages against non-typhoidal Salmonella

Haslinda W. H., . and Tang, J. Y. H. and Tuan Zainazor, T. C. (2024) Isolation and characterisation of two lytic bacteriophages against non-typhoidal Salmonella. International Food Research Journal (Malaysia), 31. pp. 1060-1075. ISSN 2231 7546

With the increasing rate of antibiotics failure, attention has been drawn to the bacteriophage as an alternative to control multidrug resistant bacteria. Herein, two Salmonella bacteriophages SH1 and SH2, capable of infecting multiple Salmonella serovars, were isolated and characterised. The phages were isolated from chicken fillet samples using the double agar overlay plaque assay by using Salmonella Hvittingfoss as a host bacterium. SH1 and SH2, the double-stranded DNA phages which belong to the family Siphoviridae, were found to have broad host range due to the capability of inhibiting 37 and 38 out of 58 Salmonella strains, which included 17 and 16 different serovars, respectively. Both phages were found to have the optimal multiplicity of infection of 1 with good pH tolerance (pH 5 - 11) and thermal stability (4 - 50°C). For the reproduction cycle, it was discovered that SH1 and SH2 have the latent periods of 20 and 10 min with average burst sizes of 59 and 52 PFU/cell, respectively. A total of 0.31 ± 0.01 and 0.32 ± 0.04 log CFU/mL viable cells of Salmonella host were significantly reduced from the initial count after treatment with SH1 and SH2 for 24 h, respectively. Moreover, SH1 and SH2 were discovered to have an effective eradication effect towards S. Hvittingfoss with a percentage of 99.74 ± 0.17% and 89.40 ± 0.29%, respectively, after 24 h treatment. The present work suggests that SH1 and SH2 could provide a good prevention and control effect against planktonic antibiotic-resistant Salmonella and its biofilms. The present work also describes the potential of both bacteriophages to be used for biocontrol of Salmonella in food owing to their features, particularly the stability and broad host range.

Full text available from: Official URL: http://www.ifrj.upm.edu.my/31%20(04)%202024/22%20-...

Abstract

With the increasing rate of antibiotics failure, attention has been drawn to the bacteriophage as an alternative to control multidrug resistant bacteria. Herein, two Salmonella bacteriophages SH1 and SH2, capable of infecting multiple Salmonella serovars, were isolated and characterised. The phages were isolated from chicken fillet samples using the double agar overlay plaque assay by using Salmonella Hvittingfoss as a host bacterium. SH1 and SH2, the double-stranded DNA phages which belong to the family Siphoviridae, were found to have broad host range due to the capability of inhibiting 37 and 38 out of 58 Salmonella strains, which included 17 and 16 different serovars, respectively. Both phages were found to have the optimal multiplicity of infection of 1 with good pH tolerance (pH 5 - 11) and thermal stability (4 - 50°C). For the reproduction cycle, it was discovered that SH1 and SH2 have the latent periods of 20 and 10 min with average burst sizes of 59 and 52 PFU/cell, respectively. A total of 0.31 ± 0.01 and 0.32 ± 0.04 log CFU/mL viable cells of Salmonella host were significantly reduced from the initial count after treatment with SH1 and SH2 for 24 h, respectively. Moreover, SH1 and SH2 were discovered to have an effective eradication effect towards S. Hvittingfoss with a percentage of 99.74 ± 0.17% and 89.40 ± 0.29%, respectively, after 24 h treatment. The present work suggests that SH1 and SH2 could provide a good prevention and control effect against planktonic antibiotic-resistant Salmonella and its biofilms. The present work also describes the potential of both bacteriophages to be used for biocontrol of Salmonella in food owing to their features, particularly the stability and broad host range.

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Item Type:	Article
AGROVOC Term:	Salmonella
AGROVOC Term:	bacteriophages
AGROVOC Term:	biofilms (microbiology)
AGROVOC Term:	isolation
AGROVOC Term:	characterization
AGROVOC Term:	control rights
AGROVOC Term:	drug resistance
AGROVOC Term:	disease eradication
Geographical Term:	Malaysia
Depositing User:	Nor Hasnita Abdul Samat
Date Deposited:	29 Apr 2026 08:24
Last Modified:	29 Apr 2026 08:24
URI:	http://webagris.upm.edu.my/id/eprint/3256

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