Citation
Siti Nur Adila H., . and Nor Azma Y., . and UNSPECIFIED (2021) Optimization of selected plant growth regulators on callus induction of Oryza sativa L. var MR 219. Food Research (Malaysia), 5 (S4). pp. 38-45. ISSN 2550-2166
Abstract
MR 219, an indica rice from a cross between MR 137 and MR 151 is a long-grain rice variety of high-yielding, good quality of shape and taste, short maturation as well as resistance to blast and bacterial leaf blight. However, it is regarded as a recalcitrant variety due to its low ratio of callogenesis and regeneration in in vitro culturing. This would be a hindrance to crop improvements such as genetic transformation and other crop improvement methods. The objective of this study was to optimize the concentrations and combinations of plant growth regulators (PGRs) which were 2,4-Dichlorophenoxyacetic acid (2,4-D), Kinetin (KIN) and Naphthalene acetic acid (NAA) on its callus induction that ranging from 0.5 to 15.0 mg/L. The callus was induced from the mature seeds of MR 219 on the Murashige and Skoog (MS) media supplemented with 4.4 g/L of MS powder with vitamins, 30.0 g/L of sucrose and 3.5 g/L of gelrite with the addition of 2,4-D, KIN and NAA in different concentrations and combinations. All media supplemented with 2,4-D had successfully induced the callus and 2.0 mg/L of 2,4-D was the best concentration for the callus induction with a 100% success rate. The addition of 15.0 mg/L of NAA, provided less time taken for callus induction with better callus morphology which resulted in fewer browning problems.
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Abstract
MR 219, an indica rice from a cross between MR 137 and MR 151 is a long-grain rice variety of high-yielding, good quality of shape and taste, short maturation as well as resistance to blast and bacterial leaf blight. However, it is regarded as a recalcitrant variety due to its low ratio of callogenesis and regeneration in in vitro culturing. This would be a hindrance to crop improvements such as genetic transformation and other crop improvement methods. The objective of this study was to optimize the concentrations and combinations of plant growth regulators (PGRs) which were 2,4-Dichlorophenoxyacetic acid (2,4-D), Kinetin (KIN) and Naphthalene acetic acid (NAA) on its callus induction that ranging from 0.5 to 15.0 mg/L. The callus was induced from the mature seeds of MR 219 on the Murashige and Skoog (MS) media supplemented with 4.4 g/L of MS powder with vitamins, 30.0 g/L of sucrose and 3.5 g/L of gelrite with the addition of 2,4-D, KIN and NAA in different concentrations and combinations. All media supplemented with 2,4-D had successfully induced the callus and 2.0 mg/L of 2,4-D was the best concentration for the callus induction with a 100% success rate. The addition of 15.0 mg/L of NAA, provided less time taken for callus induction with better callus morphology which resulted in fewer browning problems.
Additional Metadata
| Item Type: | Article |
|---|---|
| Additional Information: | 2nd International Plantation Conference (IPC2021) |
| AGROVOC Term: | Oryza sativa |
| AGROVOC Term: | rice |
| AGROVOC Term: | in vitro culture |
| AGROVOC Term: | plant growth substances |
| AGROVOC Term: | cytokinins |
| AGROVOC Term: | callus |
| AGROVOC Term: | somatic embryogenesis |
| Geographical Term: | Malaysia |
| Depositing User: | Mr. Khoirul Asrimi Md Nor |
| Date Deposited: | 29 Jan 2026 06:44 |
| Last Modified: | 29 Jan 2026 06:44 |
| URI: | http://webagris.upm.edu.my/id/eprint/3312 |
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