Detection of changes in growth, yield and genetic variation using RAPD markers among M1V2 and M1V3 generations of irradiated ginger (Zingiber officinale Roscoe)

Mohd Sharim M.A., . and Shamsiah A., . (2021) Detection of changes in growth, yield and genetic variation using RAPD markers among M1V2 and M1V3 generations of irradiated ginger (Zingiber officinale Roscoe). Food Research (Malaysia), 5 (S4). pp. 74-82. ISSN 2550-2166

The effects of gamma-rays on the growth and yield of twelve mutant lines of Zingiber officinale Roscoe from 2 varieties; namely Bentong and Tanjung Sepat were analysed for a second (M₁V₂) and a third generation (M₁V₃). Mutant rhizomes have previously been exposed to different doses (0, 5, 7, 9, 11, and 13 Gy) of gamma-rays that were propagated until the third generation. In the study, the phenotypic characteristic and genetic variation study using Randomly Amplified Polymorphic DNA (RAPD) of ginger mutant lines of M₁V₂ and M₁V₃ were analysed and compared with the first generation (M₁V₁). Parameters such as sprouting rhizome, plant height, the average number of leaves and shoots, average length and width of leaves, and average weights of rhizomes per plant for mutant lines of both varieties were collected. It was observed that the increase in dosage had a negative effect on the growth performance of plants. The number of leaves and shoots, length and width of leaves and average weight of rhizomes for both generations were decreased as the dose increased. The plants from the treatment 5 Gy dose produced the highest ginger rhizome yields of 65.45±1.35 g and 165.0±2.30 g in the Bentong and Tanjung Sepat varieties respectively, after nine months of cultivation in M₁V₂. While in M₁V₃, the rhizome yield at 5 Gy showed that the highest rhizome yields were reduced in both varieties of Bentong (45.45±1.25 g) and Tanjung Sepat (125.0±2.30 g). The PCR-based RAPD analysis showed 98.29% of polymorphism which indicated genetic variations between ginger mutant lines. The information provides an important input in determining resourceful management strategies for genetics improvement of ginger.

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Abstract

The effects of gamma-rays on the growth and yield of twelve mutant lines of Zingiber officinale Roscoe from 2 varieties; namely Bentong and Tanjung Sepat were analysed for a second (M₁V₂) and a third generation (M₁V₃). Mutant rhizomes have previously been exposed to different doses (0, 5, 7, 9, 11, and 13 Gy) of gamma-rays that were propagated until the third generation. In the study, the phenotypic characteristic and genetic variation study using Randomly Amplified Polymorphic DNA (RAPD) of ginger mutant lines of M₁V₂ and M₁V₃ were analysed and compared with the first generation (M₁V₁). Parameters such as sprouting rhizome, plant height, the average number of leaves and shoots, average length and width of leaves, and average weights of rhizomes per plant for mutant lines of both varieties were collected. It was observed that the increase in dosage had a negative effect on the growth performance of plants. The number of leaves and shoots, length and width of leaves and average weight of rhizomes for both generations were decreased as the dose increased. The plants from the treatment 5 Gy dose produced the highest ginger rhizome yields of 65.45±1.35 g and 165.0±2.30 g in the Bentong and Tanjung Sepat varieties respectively, after nine months of cultivation in M₁V₂. While in M₁V₃, the rhizome yield at 5 Gy showed that the highest rhizome yields were reduced in both varieties of Bentong (45.45±1.25 g) and Tanjung Sepat (125.0±2.30 g). The PCR-based RAPD analysis showed 98.29% of polymorphism which indicated genetic variations between ginger mutant lines. The information provides an important input in determining resourceful management strategies for genetics improvement of ginger.

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Item Type:	Article
Additional Information:	2nd International Plantation Conference (IPC2021)
AGROVOC Term:	ginger
AGROVOC Term:	mutation breeding
AGROVOC Term:	yield forecasting
AGROVOC Term:	genetic variation
AGROVOC Term:	gamma radiation
AGROVOC Term:	plant growth
AGROVOC Term:	genetic polymorphism
AGROVOC Term:	rhizomes
AGROVOC Term:	dosage
AGROVOC Term:	plant height
Geographical Term:	Malaysia
Depositing User:	Mr. Khoirul Asrimi Md Nor
Date Deposited:	05 Feb 2026 03:26
Last Modified:	05 Feb 2026 03:26
URI:	http://webagris.upm.edu.my/id/eprint/3319

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