Citation
Nabila Huda Hamdan, . and Maryam Mohamed Rehan, . and Ogita, Shinjiro and Nazariyah Yahaya, . (2024) Novel sustainable bio-fertilizer formulated with mangrove-associated bacteria enhances duckweed growth and protein content. Pertanika Journal Tropical Agricultural Science (Malaysia), 47 (3). pp. 819-841. ISSN 1511-3701
Abstract
Duckweed is a future food and a source of affordable protein that has the potential to replace animal protein. This study aims to formulate a bio-fertilizer consisting of mangrove-associated bacteria to boost the growth and protein of duckweeds as a sustainable approach to increase plant-based protein yields. The culture-depending technique was performed by using Aleksandrow agar, Pikovskaya’s agar, and Jensen agar to screen potassium-solubilizing bacteria, phosphate-solubilizing bacteria and nitrogen-fixing bacteria, respectively, from mangrove soil sediments. Mangrove-associated bacteria that are close to Acinetobacter radioresistens, Brachybacterium paraconglomeratum, and Enterobacter cloacae, which are known as nitrogen-fixing bacteria, Klebsiella quasipneumoniae, Bacillus tropicus, and Paenibacillus pasadenensis known as potassium-solubilizing bacteria, and Bacillus cereus and Bacillus thuringiensis known as phosphate-solubilizing bacteria were identified through 16S rRNA gene sequencing. After that, three sets of bio-fertilizers were randomly formulated. Each set consisted of nitrogen-fixing bacteria, potassium- and phosphate-solubilizing bacteria, as well as commercial compost as a carrier. These formulated bio-fertilizers were evaluated for plant growth promotion and protein production on duckweed plants under temperatures between 26 and 30°C. The results showed that each set of our formulated bio-fertilizer can increase the nitrogen (N), phosphorus (P), and potassium (K), duckweed growth, and protein content when compared to the control group. It indicates that bio-fertilizers formulated with mangrove-associated bacteria and high NPK contents could enhance the growth of duckweed as well as its protein content, which could supply our future plant-based protein sustainably.
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Abstract
Duckweed is a future food and a source of affordable protein that has the potential to replace animal protein. This study aims to formulate a bio-fertilizer consisting of mangrove-associated bacteria to boost the growth and protein of duckweeds as a sustainable approach to increase plant-based protein yields. The culture-depending technique was performed by using Aleksandrow agar, Pikovskaya’s agar, and Jensen agar to screen potassium-solubilizing bacteria, phosphate-solubilizing bacteria and nitrogen-fixing bacteria, respectively, from mangrove soil sediments. Mangrove-associated bacteria that are close to Acinetobacter radioresistens, Brachybacterium paraconglomeratum, and Enterobacter cloacae, which are known as nitrogen-fixing bacteria, Klebsiella quasipneumoniae, Bacillus tropicus, and Paenibacillus pasadenensis known as potassium-solubilizing bacteria, and Bacillus cereus and Bacillus thuringiensis known as phosphate-solubilizing bacteria were identified through 16S rRNA gene sequencing. After that, three sets of bio-fertilizers were randomly formulated. Each set consisted of nitrogen-fixing bacteria, potassium- and phosphate-solubilizing bacteria, as well as commercial compost as a carrier. These formulated bio-fertilizers were evaluated for plant growth promotion and protein production on duckweed plants under temperatures between 26 and 30°C. The results showed that each set of our formulated bio-fertilizer can increase the nitrogen (N), phosphorus (P), and potassium (K), duckweed growth, and protein content when compared to the control group. It indicates that bio-fertilizers formulated with mangrove-associated bacteria and high NPK contents could enhance the growth of duckweed as well as its protein content, which could supply our future plant-based protein sustainably.
Additional Metadata
| Item Type: | Article |
|---|---|
| AGROVOC Term: | proteins |
| AGROVOC Term: | fertilization |
| AGROVOC Term: | nitrogen-fixing bacteria |
| AGROVOC Term: | plant growth |
| AGROVOC Term: | protein content |
| AGROVOC Term: | nutrient uptake |
| AGROVOC Term: | sustainable agriculture |
| AGROVOC Term: | food security |
| AGROVOC Term: | soil amendments |
| Geographical Term: | Malaysia |
| Depositing User: | Ms. Azariah Hashim |
| Date Deposited: | 26 Feb 2026 06:42 |
| Last Modified: | 26 Feb 2026 06:42 |
| URI: | http://webagris.upm.edu.my/id/eprint/3382 |
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