Selection and optimisation of extraction technique for the preparation of phenolic- and flavonoid-rich extract of leafy vegetable, Coccinia grandis (Linn.) voigt

Shanmuganathan, E. and Arawwawala, L. D. A. M. and Wasana, K. G. P. and Attanayake, A. P. (2022) Selection and optimisation of extraction technique for the preparation of phenolic- and flavonoid-rich extract of leafy vegetable, Coccinia grandis (Linn.) voigt. International Food Research Journal (Malaysia), 29. pp. 1032-1042. ISSN 2231 7546

Coccinia grandis (L.) Voigt (family: Cucurbitaceae) is a popular leafy vegetable in Sri Lankan diet. C. grandis is high in phenolics and flavonoids. The present work attempted to determine a suitable extraction technique, and further optimise it to obtain phenolicand flavonoid-rich extract from C. grandis leaves, with an aim at developing a nutraceutical targeting the dietary management of diabetes mellitus. Acetone extraction (AE), methanol extraction (ME), pre-warmed water extraction (PWE), electric shake extraction (ESE), reflux extraction (RE), ultrasonication with water (UEw), ultrasonication with ethanol (UEe), ultrasonic assisted-reflux extraction (URE), and reflux assistedultrasonic extraction (RUE) were chosen as the extraction techniques. URE was selected as a satisfactory extraction technique for further optimisation for the preparation of phenolic- and flavonoid-rich extract based on the contents of phenolics (32.97 ± 0.41 mg of equivalent gallic acid/g of extract) and flavonoids (4.50 ± 0.04 mg equivalent quercetin/g of extract). The highest yield of 32.8% was obtained by the URE technique. The optimal extraction conditions for URE were determined with an ultrasonic time of 19 min, refluxing time of 168 min, and liquid:solid ratio of 16.4 mL/g. This is the first attempt to investigate the selection and optimisation of an extraction technique for obtaining phenolic- and flavonoid-rich extract from C. grandis leaves. The present findings would be useful in the development of a commercially viable nutraceutical using a phenolic- and flavonoid-rich extract of C. grandis.

Full text available from: Official URL: http://www.ifrj.upm.edu.my/29%20(05)%202022/06%20-...

Abstract

Coccinia grandis (L.) Voigt (family: Cucurbitaceae) is a popular leafy vegetable in Sri Lankan diet. C. grandis is high in phenolics and flavonoids. The present work attempted to determine a suitable extraction technique, and further optimise it to obtain phenolicand flavonoid-rich extract from C. grandis leaves, with an aim at developing a nutraceutical targeting the dietary management of diabetes mellitus. Acetone extraction (AE), methanol extraction (ME), pre-warmed water extraction (PWE), electric shake extraction (ESE), reflux extraction (RE), ultrasonication with water (UEw), ultrasonication with ethanol (UEe), ultrasonic assisted-reflux extraction (URE), and reflux assistedultrasonic extraction (RUE) were chosen as the extraction techniques. URE was selected as a satisfactory extraction technique for further optimisation for the preparation of phenolic- and flavonoid-rich extract based on the contents of phenolics (32.97 ± 0.41 mg of equivalent gallic acid/g of extract) and flavonoids (4.50 ± 0.04 mg equivalent quercetin/g of extract). The highest yield of 32.8% was obtained by the URE technique. The optimal extraction conditions for URE were determined with an ultrasonic time of 19 min, refluxing time of 168 min, and liquid:solid ratio of 16.4 mL/g. This is the first attempt to investigate the selection and optimisation of an extraction technique for obtaining phenolic- and flavonoid-rich extract from C. grandis leaves. The present findings would be useful in the development of a commercially viable nutraceutical using a phenolic- and flavonoid-rich extract of C. grandis.

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Item Type:	Article
AGROVOC Term:	Coccinia grandis
AGROVOC Term:	phenolic content
AGROVOC Term:	flavonoids
AGROVOC Term:	extraction
AGROVOC Term:	ultrasonics
AGROVOC Term:	statistical analysis > statistical analysis Prefer using statistical methodsstatistical methods
AGROVOC Term:	novel products > novel products Prefer using new productsnew products
Geographical Term:	Sri Lanka
Depositing User:	Nor Hasnita Abdul Samat
Date Deposited:	05 Sep 2024 06:50
Last Modified:	05 Sep 2024 06:50
URI:	http://webagris.upm.edu.my/id/eprint/34

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