The effects of leds light spectra in Labisia pumila var. Alata

Nurul Hanis Yahaya, . and Siti Suhaila A. Rahman, . and Mohd Syahiran Sulaiman, . and Nurnadiah Roslan, . and Mohd Zaki Abdullah, . (2022) The effects of leds light spectra in Labisia pumila var. Alata. International Journal of Agriculture, Forestry and Plantation (Malaysia), 12. pp. 83-90. ISSN 2462-1757

Labisia pumila var. alata is a high-value herbal commodity with a promising future in the global herbal and pharmaceutical industry. As the application of L. pumila is being explored and new products invented, increase in demand for raw materials are increasing exponentially. Therefore, production needs to be increased to avoid an insufficient supply of the L. pumila raw materials to support the ever-growing industry. Labisia spp. can be propagated through in vitro culture; however, there is still some improvement that could accelerate the production in vitro. Apart from the medium culture, carbon source, and plant growth regulators; environmental factors are the main issues regarding the lack of protocol reproducibility among plant tissue culture. Light quantity and quality, in particular, are among the most important factors as they can affect plant growth and development with other environmental parameters and cultivation factors in determining plant behaviour. With the advent of light-emitting diode (LED) technology, impressive advances have been achieved in environmental controls and morphogenetic responses in many plant species; however, seldom present the spectral quality of the light sources used in L. pumila. Hence, the objective of this study is to evaluate the effects of different LEDs light spectra (100% white (control), 80% red and 20% blue, 50% red and 50% blue, 100% red and 100% blue) on morphological development in L. pumila var. alata using two different containers (glass jar and magenta box). After five weeks, results show the highest percentage of callus formation from explants incubated under white LED treatment in glass jars with 100% callus formation, whereas cultures from magenta box yielded at 33.3%. However, explants treated under red and blue LEDs light (either monochromatic or combinations) resulted in a small percentage of callus formation compared to control, with <40% and <25% of callus formation in a glass jar and magenta box containers, respectively. The explants also experienced dryness due to heat released under the LEDs light treatments except in control. No shoot formation was observed in all cultures. The result indicated that glass jars show the best growth of explants while higher wattage light caused high temperature which led to irreversible damage to the explants’ function and development in L. pumila var. alata.

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Abstract

Labisia pumila var. alata is a high-value herbal commodity with a promising future in the global herbal and pharmaceutical industry. As the application of L. pumila is being explored and new products invented, increase in demand for raw materials are increasing exponentially. Therefore, production needs to be increased to avoid an insufficient supply of the L. pumila raw materials to support the ever-growing industry. Labisia spp. can be propagated through in vitro culture; however, there is still some improvement that could accelerate the production in vitro. Apart from the medium culture, carbon source, and plant growth regulators; environmental factors are the main issues regarding the lack of protocol reproducibility among plant tissue culture. Light quantity and quality, in particular, are among the most important factors as they can affect plant growth and development with other environmental parameters and cultivation factors in determining plant behaviour. With the advent of light-emitting diode (LED) technology, impressive advances have been achieved in environmental controls and morphogenetic responses in many plant species; however, seldom present the spectral quality of the light sources used in L. pumila. Hence, the objective of this study is to evaluate the effects of different LEDs light spectra (100% white (control), 80% red and 20% blue, 50% red and 50% blue, 100% red and 100% blue) on morphological development in L. pumila var. alata using two different containers (glass jar and magenta box). After five weeks, results show the highest percentage of callus formation from explants incubated under white LED treatment in glass jars with 100% callus formation, whereas cultures from magenta box yielded at 33.3%. However, explants treated under red and blue LEDs light (either monochromatic or combinations) resulted in a small percentage of callus formation compared to control, with <40% and <25% of callus formation in a glass jar and magenta box containers, respectively. The explants also experienced dryness due to heat released under the LEDs light treatments except in control. No shoot formation was observed in all cultures. The result indicated that glass jars show the best growth of explants while higher wattage light caused high temperature which led to irreversible damage to the explants’ function and development in L. pumila var. alata.

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Item Type:	Article
AGROVOC Term:	pharmaceutical industry
AGROVOC Term:	plant propagation
AGROVOC Term:	in vitro culture
AGROVOC Term:	containers
AGROVOC Term:	temperature
AGROVOC Term:	plant growth
Geographical Term:	Malaysia
Depositing User:	Mr. Khoirul Asrimi Md Nor
Date Deposited:	20 May 2026 06:26
Last Modified:	20 May 2026 06:26
URI:	http://webagris.upm.edu.my/id/eprint/3906

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