Effect of storage on the biological activity of misai kucing leaves and antibacterial activity of misai kucing-seaweed edible films

Raja Arief Deli Raja Nasharuddin, . and Noor Ismawaty Nordin, . and Siah Watt Moey, . and Syahida Maarof, . (2022) Effect of storage on the biological activity of misai kucing leaves and antibacterial activity of misai kucing-seaweed edible films. International Journal of Agriculture, Forestry and Plantation (Malaysia), 12. pp. 112-120. ISSN 2462-1757

Misai kucing or Orthosiphon stamineus Benth is widely planted in Southeast Asia including Tropical Australia as herbal remedies known for its medicinal benefits. Our preliminary studies have shown leaves methanolic extract of misai kucing contained good antibacterial activity against Staphylococcus aureus, Bacillus cereus and Vibrio parahaemolyticus at week 10 of maturity stages, from peat soil and mineral soil at MARDI Pontian and MARDI Serdang field plots, respectively. We were interested in investigating whether misai kucing leaves could have the same biological activity after prolonged storage of misai kucing powder at room temperature and to determine the antibacterial activity of misai kucing extract incorporated into seaweed-based edible films. Interestingly, the leaves extract of misai kucing from MARDI Pontian and Serdang still possessed strong antibacterial activity after three years storage with inhibition zone of 12.1 mm; 12.5 mm and MIC value 1.56 mg/mL; 1.56 mg/mL against S. aureus, respectively. Following to that, seaweed edible films incorporated with misai kucing extract of one, five and ten times their MICs were tested for the antibacterial effect against test microorganisms. The inhibitory effects were observed promising against S. aureus at higher concentrations than their MICs, and samples from MARDI Serdang gave better results in contrast to MARDI Pontian (p<0.05) at ten times MIC. Overall, the study indicated that misai kucing leaves could be used even after storage for prolonged time periods and misai kucing-seaweed films have the antibacterial potential as natural food safety synergistic applications in bio-based food packaging.

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Abstract

Misai kucing or Orthosiphon stamineus Benth is widely planted in Southeast Asia including Tropical Australia as herbal remedies known for its medicinal benefits. Our preliminary studies have shown leaves methanolic extract of misai kucing contained good antibacterial activity against Staphylococcus aureus, Bacillus cereus and Vibrio parahaemolyticus at week 10 of maturity stages, from peat soil and mineral soil at MARDI Pontian and MARDI Serdang field plots, respectively. We were interested in investigating whether misai kucing leaves could have the same biological activity after prolonged storage of misai kucing powder at room temperature and to determine the antibacterial activity of misai kucing extract incorporated into seaweed-based edible films. Interestingly, the leaves extract of misai kucing from MARDI Pontian and Serdang still possessed strong antibacterial activity after three years storage with inhibition zone of 12.1 mm; 12.5 mm and MIC value 1.56 mg/mL; 1.56 mg/mL against S. aureus, respectively. Following to that, seaweed edible films incorporated with misai kucing extract of one, five and ten times their MICs were tested for the antibacterial effect against test microorganisms. The inhibitory effects were observed promising against S. aureus at higher concentrations than their MICs, and samples from MARDI Serdang gave better results in contrast to MARDI Pontian (p<0.05) at ten times MIC. Overall, the study indicated that misai kucing leaves could be used even after storage for prolonged time periods and misai kucing-seaweed films have the antibacterial potential as natural food safety synergistic applications in bio-based food packaging.

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Item Type:	Article
AGROVOC Term:	leaves
AGROVOC Term:	edible films
AGROVOC Term:	seaweeds
AGROVOC Term:	powders
AGROVOC Term:	storage
AGROVOC Term:	extraction
AGROVOC Term:	incorporation
AGROVOC Term:	food packaging
AGROVOC Term:	Staphylococcus aureus
AGROVOC Term:	Bacillus cereus
Geographical Term:	Malaysia
Depositing User:	Mr. Khoirul Asrimi Md Nor
Date Deposited:	20 May 2026 07:59
Last Modified:	20 May 2026 07:59
URI:	http://webagris.upm.edu.my/id/eprint/3914

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