Incorporation of plant preservative mixture (PPMTM) to improve surface sterilization of Tinospora crispa in vitro

Haliza Ismail, . and Norhasnida Hassan, . and Nazirah Abdullah, . (2023) Incorporation of plant preservative mixture (PPMTM) to improve surface sterilization of Tinospora crispa in vitro. International Journal of Agriculture, Forestry and Plantation (Malaysia), 13. pp. 154-157. ISSN 2462-1757

Tinospora crispa (L) Hook. F. & Thomson is locally known as Patawali and belongs to Menispermaceae family. It is mostly found in Asia and Africa and well known for its traditional medicinal properties. It is used as traditional medicine against jaundice, rheumatism, fever, malaria, diabetes, hypertension and can reduce body temperature. Micropropagation techniques can be an alternative method for mass propagation of this species particularly when using samples from selected clones. Young and green nodal segments are more recommendable to use in this study as they are more juvenile and easier to be micropropagated than older part of the plants. The purpose of this paper is to provide information regarding the incorporation of biocide PPMTM for surface sterilization of young nodal segments of Tinospora crispa to improve the percentage of clean explants obtained.

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Abstract

Tinospora crispa (L) Hook. F. & Thomson is locally known as Patawali and belongs to Menispermaceae family. It is mostly found in Asia and Africa and well known for its traditional medicinal properties. It is used as traditional medicine against jaundice, rheumatism, fever, malaria, diabetes, hypertension and can reduce body temperature. Micropropagation techniques can be an alternative method for mass propagation of this species particularly when using samples from selected clones. Young and green nodal segments are more recommendable to use in this study as they are more juvenile and easier to be micropropagated than older part of the plants. The purpose of this paper is to provide information regarding the incorporation of biocide PPMTM for surface sterilization of young nodal segments of Tinospora crispa to improve the percentage of clean explants obtained.

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Item Type:	Article
AGROVOC Term:	medicinal plants
AGROVOC Term:	nematodes
AGROVOC Term:	micropropagation
AGROVOC Term:	in vitro
AGROVOC Term:	biocides
Geographical Term:	Malaysia
Depositing User:	Mr. Khoirul Asrimi Md Nor
Date Deposited:	10 Jun 2026 12:41
Last Modified:	10 Jun 2026 12:41
URI:	http://webagris.upm.edu.my/id/eprint/3999

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