Complete plantlet generation and acclimatization of the medicinal herb Zingiber officinale var. rubrum

Nazirah Abdullah, . and Nor Hasnida Hassan, . and Haliza Ismail, . (2023) Complete plantlet generation and acclimatization of the medicinal herb Zingiber officinale var. rubrum. International Journal of Agriculture, Forestry and Plantation (Malaysia), 13. pp. 222-226. ISSN 2462-1757

Zingiber officinale var. rubrum, also known as red ginger, is a perennial herb that is widely used in traditional medicine. The rhizome extract of Z. officinale var. rubrum has been reported to have antioxidant activity, anti-inflammatory activity, anti- nausea/antiemetic activity, anti-bacterial activity, cytotoxic activity, and antidiabetic activity. Using tissue culture techniques, the mass propagation of red ginger is studied. In a previous experiment, the red ginger shoot multiplication medium was determined, which is Murashige and Skoog (MS) media with 1.0 mg/L BAP supplementation. Even though the plantlets were able to establish a root system during the shoot multiplication stage, an experiment to study the effect of rooting hormone on the red ginger was conducted. The effects of two types of auxin, IBA and NAA, at different concentrations on the root induction of red ginger have been studied. Plantlets, measuring 3–4 cm in length, were cultured in MS media supplemented with IBA or NAA at different concentrations. Media without hormones was used as a control. After 1 week, all plantlets generated a root system in all medium experiments, with the highest number of roots per explant in the medium without hormone. The complete plantlets were acclimatized in four different media in a weaning chamber. The mixture of top soil, baked soil, and peat moss (2:1:1) had the highest survival rate, with 100% survival, and the mixture of peat moss and sand (1:1) had the lowest, with 50% survival. The plantlets that survived were placed in a polybag filled with mixed soil, top soil: baked soil and peat moss (2:1:1), placed under 50% shade, and watered three times daily. After 2 months, the average growth is 5 cm. The protocol developed can be applied for mass production of Z. officinale var. rubrum.

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Abstract

Zingiber officinale var. rubrum, also known as red ginger, is a perennial herb that is widely used in traditional medicine. The rhizome extract of Z. officinale var. rubrum has been reported to have antioxidant activity, anti-inflammatory activity, anti- nausea/antiemetic activity, anti-bacterial activity, cytotoxic activity, and antidiabetic activity. Using tissue culture techniques, the mass propagation of red ginger is studied. In a previous experiment, the red ginger shoot multiplication medium was determined, which is Murashige and Skoog (MS) media with 1.0 mg/L BAP supplementation. Even though the plantlets were able to establish a root system during the shoot multiplication stage, an experiment to study the effect of rooting hormone on the red ginger was conducted. The effects of two types of auxin, IBA and NAA, at different concentrations on the root induction of red ginger have been studied. Plantlets, measuring 3–4 cm in length, were cultured in MS media supplemented with IBA or NAA at different concentrations. Media without hormones was used as a control. After 1 week, all plantlets generated a root system in all medium experiments, with the highest number of roots per explant in the medium without hormone. The complete plantlets were acclimatized in four different media in a weaning chamber. The mixture of top soil, baked soil, and peat moss (2:1:1) had the highest survival rate, with 100% survival, and the mixture of peat moss and sand (1:1) had the lowest, with 50% survival. The plantlets that survived were placed in a polybag filled with mixed soil, top soil: baked soil and peat moss (2:1:1), placed under 50% shade, and watered three times daily. After 2 months, the average growth is 5 cm. The protocol developed can be applied for mass production of Z. officinale var. rubrum.

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Item Type:	Article
AGROVOC Term:	Zingiber officinale
AGROVOC Term:	ginger
AGROVOC Term:	medicinal plants
AGROVOC Term:	plant propagation
AGROVOC Term:	tissue culture
AGROVOC Term:	acclimatization
AGROVOC Term:	hormones
AGROVOC Term:	auxins
AGROVOC Term:	culture media
AGROVOC Term:	insect growth regulators
Geographical Term:	Malaysia
Depositing User:	Mr. Khoirul Asrimi Md Nor
Date Deposited:	11 Jun 2026 03:17
Last Modified:	11 Jun 2026 03:17
URI:	http://webagris.upm.edu.my/id/eprint/4020

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