Preparation, characterisation, and antioxidant activity of honeysuckle polysaccharide nano-selenium

Sun, J. R. and Li, J. L. and Yuan, J. F. and Yao, L. L. and You, F. F. and Wang, D. H. and Gu, S. B. (2024) Preparation, characterisation, and antioxidant activity of honeysuckle polysaccharide nano-selenium. International Food Research Journal (Malaysia), 31 (6). pp. 1580-1590. ISSN 2231 7546

Using honeysuckle as raw material, honeysuckle polysaccharide (HP) was obtained by water extraction and alcohol precipitation, and polysaccharide nano-selenium (HP-SeNPs) was prepared by HNO3-Na2SeO4 method. The structure of HP, before and after selenisation, was characterised by gas chromatography-mass spectrometry, ultraviolet (UV), Fourier-transform infrared (FT-IR), scanning electron microscopy (SEM), and Xray diffraction (XRD), and the antioxidant activity was studied. The results showed that the content of Se in HP-SeNPs could reach 10.45%. The HP-SeNPs primarily consisted of glucose and mannose, with a molecular weight of 73.8 kDa. The characterisation results revealed that HP-SeNPs exhibited a novel absorption peak at approximately 334 nm in the UV spectrum; distinct absorption peaks were observed at 1079.66 and 619.21 cm-1 in the FT-IR spectrum, while a broad diffraction peak appeared between 19° and 25° in the XRD pattern. SEM further demonstrated that HP-SeNPs formed particle-like surface structures, altering the surface morphology of polysaccharides. These findings collectively indicated the successful preparation of HP-SeNPs. The scavenging ability of HP-SeNPs to DPPH, ·OH, and ABTS free radicals was concentration-dependent, and the highest scavenging rates were 53.85, 62.55, and 49.37%, which were significantly higher than HP. This indicated that selenium modification could outstandingly improve the antioxidant activity of HP.

Full text available from: Official URL: http://www.ifrj.upm.edu.my/31%20(06)%202024/16%20-...

Abstract

Using honeysuckle as raw material, honeysuckle polysaccharide (HP) was obtained by water extraction and alcohol precipitation, and polysaccharide nano-selenium (HP-SeNPs) was prepared by HNO3-Na2SeO4 method. The structure of HP, before and after selenisation, was characterised by gas chromatography-mass spectrometry, ultraviolet (UV), Fourier-transform infrared (FT-IR), scanning electron microscopy (SEM), and Xray diffraction (XRD), and the antioxidant activity was studied. The results showed that the content of Se in HP-SeNPs could reach 10.45%. The HP-SeNPs primarily consisted of glucose and mannose, with a molecular weight of 73.8 kDa. The characterisation results revealed that HP-SeNPs exhibited a novel absorption peak at approximately 334 nm in the UV spectrum; distinct absorption peaks were observed at 1079.66 and 619.21 cm-1 in the FT-IR spectrum, while a broad diffraction peak appeared between 19° and 25° in the XRD pattern. SEM further demonstrated that HP-SeNPs formed particle-like surface structures, altering the surface morphology of polysaccharides. These findings collectively indicated the successful preparation of HP-SeNPs. The scavenging ability of HP-SeNPs to DPPH, ·OH, and ABTS free radicals was concentration-dependent, and the highest scavenging rates were 53.85, 62.55, and 49.37%, which were significantly higher than HP. This indicated that selenium modification could outstandingly improve the antioxidant activity of HP.

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Item Type:	Article
AGROVOC Term:	polysaccharides
AGROVOC Term:	selenium
AGROVOC Term:	extraction
AGROVOC Term:	site preparation
AGROVOC Term:	characterization
AGROVOC Term:	nanotechnology
AGROVOC Term:	free radicals
AGROVOC Term:	scanning electron microscopy
AGROVOC Term:	antioxidant properties
Geographical Term:	China
Uncontrolled Keywords:	honeysuckle, polysaccharide, selenide modification, characterisation, antioxidant activity
Depositing User:	Nor Hasnita Abdul Samat
Date Deposited:	14 May 2026 08:41
Last Modified:	14 May 2026 08:41
URI:	http://webagris.upm.edu.my/id/eprint/4076

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