Structural alteration of oil palm empty fruit bunch by peracetic acid combined with ultrasonic pretreatment for xylanase production by Neosartorya sp.

Poludasu, Rama Mohan and Cheirsilp, Benjamas and Boonsawang, Piyarat (2024) Structural alteration of oil palm empty fruit bunch by peracetic acid combined with ultrasonic pretreatment for xylanase production by Neosartorya sp. Journal of Oil Palm Research (Malaysia), 36 (4). pp. 674-686. ISSN 2811-4701

The excess of oil palm empty fruit bunches (OPEFB) has caused significant ecological issues. Combined peracetic acid (PAA) and ultrasonication were used for OPEFB pretreatment. PAA solutions of varying strengths and duration of ultrasonication at a frequency of 37 kHz at 60°C were examined for OPEFB pretreatment. At 10% PAA for 60 min, the highest levels of cellulose (52.0 ± 0.2%), hemicellulose (39.5 ± 0.2%), and decreased lignin (11.3 ± 0.4%) were achieved. In the ideal pretreatment condition, the highest glucose levels (28 mg g-¹) and xylose (19 mg g-¹) was observed. Under a scanning electron microscope (SEM), a significant structural change in OPEFB before and after pretreatment was discovered. FTIR of OPEFB fibre had an absorption peak at 1727 cm-¹, which relates to the hemicellulose’s C=O stretching. From the XRD studies, both crystalline and semi-crystalline zones may be seen in the diffractogram curves of OPEFB cellulose. Neosartorya sp. BCC 18065 produced more xylanase (92 ± 0.03 IU g-¹) on pretreated OPEFB compared to untreated OPEFB (61 ± 0.01 IU g-¹). With more xylanase enzymes present during fermentation, xylose production was enhanced. The results demonstrate that structural changes in OPEFB can be changed by PAA-assisted ultrasonication pretreatment, which also improves enzymatic hydrolysis and saccharification.

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Abstract

The excess of oil palm empty fruit bunches (OPEFB) has caused significant ecological issues. Combined peracetic acid (PAA) and ultrasonication were used for OPEFB pretreatment. PAA solutions of varying strengths and duration of ultrasonication at a frequency of 37 kHz at 60°C were examined for OPEFB pretreatment. At 10% PAA for 60 min, the highest levels of cellulose (52.0 ± 0.2%), hemicellulose (39.5 ± 0.2%), and decreased lignin (11.3 ± 0.4%) were achieved. In the ideal pretreatment condition, the highest glucose levels (28 mg g-¹) and xylose (19 mg g-¹) was observed. Under a scanning electron microscope (SEM), a significant structural change in OPEFB before and after pretreatment was discovered. FTIR of OPEFB fibre had an absorption peak at 1727 cm-¹, which relates to the hemicellulose’s C=O stretching. From the XRD studies, both crystalline and semi-crystalline zones may be seen in the diffractogram curves of OPEFB cellulose. Neosartorya sp. BCC 18065 produced more xylanase (92 ± 0.03 IU g-¹) on pretreated OPEFB compared to untreated OPEFB (61 ± 0.01 IU g-¹). With more xylanase enzymes present during fermentation, xylose production was enhanced. The results demonstrate that structural changes in OPEFB can be changed by PAA-assisted ultrasonication pretreatment, which also improves enzymatic hydrolysis and saccharification.

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Item Type:	Article
AGROVOC Term:	oil palms
AGROVOC Term:	biomass
AGROVOC Term:	cellulose
AGROVOC Term:	hemicellulose
AGROVOC Term:	lignins
AGROVOC Term:	production
AGROVOC Term:	hydrolysis
AGROVOC Term:	osmotic pretreatment of seed
AGROVOC Term:	xylose
AGROVOC Term:	glucose
Geographical Term:	Thailand
Uncontrolled Keywords:	characterisation, OPEFB, peracetic acid, ultrasonication, xylanase
Depositing User:	Nor Hasnita Abdul Samat
Date Deposited:	15 May 2026 04:49
Last Modified:	15 May 2026 04:49
URI:	http://webagris.upm.edu.my/id/eprint/4106

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