Citation
Kazeem, Muinat Olanike and Abbasiliasi, Sahar and Tan, Joo Shun and Azhari Samsu Baharuddin, . and Nor’ Aini Abdul Rahman, . (2025) Primary recovery of carboxymethyl cellulase from thermophilic Bacillus licheniformis 2D55 using an aqueous two-phase system. Pertanika Journal of Science & Technology, 33 (1). 1 -19. ISSN 2231-8526
Abstract
This study uses an aqueous two-phase system developed from a polymer and salt to purify a thermostable carboxymethyl cellulase (CMCase) produced by thermophilic Bacillus licheniformis 2D55. The effects of system parameters, such as polyethene glycol (PEG) molar mass, salt concentration, crude load, NaCl concentration and pH on partitioning and recovery efficiency, are evaluated. Sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) is used to determine the purity of the CMCase. The enzyme is successfully purified, achieving a 10.9-fold purification and 86.62% yield. The maximum purification condition is achieved in ATPS comprising 20.5% PEG 8000/15% sodium citrate, with a crude load of 17% (w/w), NaCl of 1.0% (w/w) and pH at 7.0. Under these conditions, a partition co-efficient of 0.21 is observed, indicating that CMCase preferentially partitions to the bottom phase. These results demonstrate the potential of ATPS for the purification of thermostable CMCase from the fermentation broth of thermophilic Bacillus licheniformis 2D55.
Download File
Official URL: http://pertanika2.upm.edu.my/resources/files/Perta...
|
![[img]](http://webagris.upm.edu.my/style/images/fileicons/text.png)
Abstract
This study uses an aqueous two-phase system developed from a polymer and salt to purify a thermostable carboxymethyl cellulase (CMCase) produced by thermophilic Bacillus licheniformis 2D55. The effects of system parameters, such as polyethene glycol (PEG) molar mass, salt concentration, crude load, NaCl concentration and pH on partitioning and recovery efficiency, are evaluated. Sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) is used to determine the purity of the CMCase. The enzyme is successfully purified, achieving a 10.9-fold purification and 86.62% yield. The maximum purification condition is achieved in ATPS comprising 20.5% PEG 8000/15% sodium citrate, with a crude load of 17% (w/w), NaCl of 1.0% (w/w) and pH at 7.0. Under these conditions, a partition co-efficient of 0.21 is observed, indicating that CMCase preferentially partitions to the bottom phase. These results demonstrate the potential of ATPS for the purification of thermostable CMCase from the fermentation broth of thermophilic Bacillus licheniformis 2D55.
Additional Metadata
| Item Type: | Article |
|---|---|
| AGROVOC Term: | cellulase |
| AGROVOC Term: | enzymes |
| AGROVOC Term: | purification |
| AGROVOC Term: | heat recovery |
| AGROVOC Term: | fermentation |
| AGROVOC Term: | Bacillus licheniformis |
| AGROVOC Term: | thermophilic microorganisms |
| AGROVOC Term: | yields |
| AGROVOC Term: | purification |
| Geographical Term: | Malaysia |
| Uncontrolled Keywords: | Aqueous two-phase system, Bacillus licheniformis 2D55, carboxymethyl cellulase, enzyme, partial purification, polyethene glycol |
| Depositing User: | Ms. Azariah Hashim |
| Date Deposited: | 17 Aug 2026 03:51 |
| Last Modified: | 17 Aug 2026 03:51 |
| URI: | http://webagris.upm.edu.my/id/eprint/4348 |
Actions (login required)
 |
View Item |
