Effect of sea surface temperature on catalase and glutathione S-transferase activities in scleractinian coral Acropora digitifera from Pulau Bidong, Terengganu

Nur Atiqah Maznan, . and Muhammad Arif Samshuri, . and Siti Nurtahirah Jaafar, . (2024) Effect of sea surface temperature on catalase and glutathione S-transferase activities in scleractinian coral Acropora digitifera from Pulau Bidong, Terengganu. Journal of Sustainability Science and Management (Malaysia), 19 (10). pp. 25-38. ISSN 2672-7226

Natural exposure of coral reefs to stressors induces an oxidative stress response. In Malaysia, biomarkers used to assess this response are limited. This study investigated the activities of the antioxidant enzymes glutathione S-transferase (GST) and catalase (CAT) in the scleractinian coral Acropora digitifera across three separate sites in Pulau Bidong, Terengganu during March, May, July, and September 2018 to understand the variations in these enzyme activities. Fragments of corals were airbrushed to remove tissue, disrupted to release cytosol, and the enzyme activities were assayed. The result demonstrated variation in GST activities with the highest level in May (p < 0.05) and in contrast, there was no significant difference in CAT activities between different sampling months and sites (p > 0.05). In addition, A. digitifera collected in Pantai Pasir Cina (PPC) showed the highest antioxidant activities for both enzymes (GST, 19.6 ± 5.10 μmol/min/mg protein; CAT, 14.6 ± 3.40 U/mg protein), suggesting corals in this location are responding to oxidative stress. These values are lower than previously reported for A. digitifera in Pulau Bidong, indicating a stable and adequate baseline level of antioxidant enzyme activities. These findings provide valuable insights and crucial information for future reference.

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Abstract

Natural exposure of coral reefs to stressors induces an oxidative stress response. In Malaysia, biomarkers used to assess this response are limited. This study investigated the activities of the antioxidant enzymes glutathione S-transferase (GST) and catalase (CAT) in the scleractinian coral Acropora digitifera across three separate sites in Pulau Bidong, Terengganu during March, May, July, and September 2018 to understand the variations in these enzyme activities. Fragments of corals were airbrushed to remove tissue, disrupted to release cytosol, and the enzyme activities were assayed. The result demonstrated variation in GST activities with the highest level in May (p < 0.05) and in contrast, there was no significant difference in CAT activities between different sampling months and sites (p > 0.05). In addition, A. digitifera collected in Pantai Pasir Cina (PPC) showed the highest antioxidant activities for both enzymes (GST, 19.6 ± 5.10 μmol/min/mg protein; CAT, 14.6 ± 3.40 U/mg protein), suggesting corals in this location are responding to oxidative stress. These values are lower than previously reported for A. digitifera in Pulau Bidong, indicating a stable and adequate baseline level of antioxidant enzyme activities. These findings provide valuable insights and crucial information for future reference.

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Item Type:	Article
AGROVOC Term:	Acropora florida
AGROVOC Term:	enzyme activity
AGROVOC Term:	sampling
AGROVOC Term:	sea surface temperature
AGROVOC Term:	oxidative stress
AGROVOC Term:	catalase
AGROVOC Term:	biomarkers
Geographical Term:	Malaysia
Depositing User:	Mr. Khoirul Asrimi Md Nor
Date Deposited:	18 Jun 2026 07:51
Last Modified:	18 Jun 2026 07:51
URI:	http://webagris.upm.edu.my/id/eprint/4361

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