Critical review of methods used in published studies of susceptibility of Vibrio spp.; lessons to be learnt

Smith, Peter and Egan, Sarah (2020) Critical review of methods used in published studies of susceptibility of Vibrio spp.; lessons to be learnt. Asian Fisheries Science Journal (Malaysia), 33. pp. 22-26. ISSN 2073-3720

The World Health Organization, the World Organisation for Animal Health, and the Food and Agriculture Organization of the United Nations recognises that the threat posed by the emergence of resistance to antimicrobials must be addressed using a One Health approach. To quantify the contribution of aquaculture to this global problem, it is essential that we generate data on the antimicrobial susceptibility of bacteria isolated from aquatic animals that is of sufficient quality. This paper presents a review of the quality of the data presented in 182 published papers on the antimicrobial susceptibility of various non-cholera Vibrio species isolated from aquatic environments. This review revealed that serious shortcomings either in the performance of the susceptibility tests or in the reporting of those tests occurred with an alarmingly high frequency. The majority of studies failed to provide sufficient details of the testing protocols they used and only a small percentage of the studies provided explicit evidence that they had used standardised susceptibility protocols. Although 203 studies reported frequencies of resistance in the isolates they studied, 185 of them either did not provided the criteria they used to determine resistance, used criteria that had not been validated or used criteria that were inappropriate. As a result of these shortcomings, it is difficult or impossible to compare the data that these papers have presented. It is argued that adopting a few simple rules in the design and reporting of susceptibility studies would, at little cost or effort, result in the production of papers that could make a significant contribution to our understanding of the issues involved in the use of antimicrobial agents in aquaculture.

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Abstract

The World Health Organization, the World Organisation for Animal Health, and the Food and Agriculture Organization of the United Nations recognises that the threat posed by the emergence of resistance to antimicrobials must be addressed using a One Health approach. To quantify the contribution of aquaculture to this global problem, it is essential that we generate data on the antimicrobial susceptibility of bacteria isolated from aquatic animals that is of sufficient quality. This paper presents a review of the quality of the data presented in 182 published papers on the antimicrobial susceptibility of various non-cholera Vibrio species isolated from aquatic environments. This review revealed that serious shortcomings either in the performance of the susceptibility tests or in the reporting of those tests occurred with an alarmingly high frequency. The majority of studies failed to provide sufficient details of the testing protocols they used and only a small percentage of the studies provided explicit evidence that they had used standardised susceptibility protocols. Although 203 studies reported frequencies of resistance in the isolates they studied, 185 of them either did not provided the criteria they used to determine resistance, used criteria that had not been validated or used criteria that were inappropriate. As a result of these shortcomings, it is difficult or impossible to compare the data that these papers have presented. It is argued that adopting a few simple rules in the design and reporting of susceptibility studies would, at little cost or effort, result in the production of papers that could make a significant contribution to our understanding of the issues involved in the use of antimicrobial agents in aquaculture.

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Item Type:	Article
AGROVOC Term:	Vibrio
AGROVOC Term:	aquaculture
AGROVOC Term:	antimicrobial resistance
AGROVOC Term:	analysis
AGROVOC Term:	laboratory experimentation
AGROVOC Term:	microbiologists
AGROVOC Term:	research support
Geographical Term:	Ireland
Depositing User:	Nor Hasnita Abdul Samat
Date Deposited:	27 Mar 2025 06:30
Last Modified:	27 Mar 2025 06:30
URI:	http://webagris.upm.edu.my/id/eprint/512

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