Chemopreventive and immunoadjuvant properties of standardised edible bird’s nest extract on human breast cancer cell line

Lee, T. H. and Y. Maruthai, . and N. H. Abd Aziz, . and Chua, K. H. and N. Hamdan, . and Lee, C. H. and N. A. Azmi, . (2023) Chemopreventive and immunoadjuvant properties of standardised edible bird’s nest extract on human breast cancer cell line. International Food Research Journal (Malaysia), 30. pp. 472-486. ISSN 2231 7546

The present work investigated the chemopreventive and immunoadjuvant properties of edible bird’s nest (EBN) extract on breast cancer cell line (MCF-7). Specifically, the cytotoxicity level of EBN extracts (HMG, EHMG, pHMG) against MCF-7, human immune cells of cytotoxic T cells, and monocytes (CD8+ and CD14+) were evaluated by measuring the production of pro-apoptotic and anti-apoptotic molecules released in single and co-culture of MCF-7, CD8+, and CD14+ cells, before and after EBN treatment. The highest cytotoxic effect towards MCF-7 using IC50 of 15 µg/mL was demonstrated by HMG but no effects on CD8+ and CD14+, with cell viability of more than 90%. At the mRNA level, activated CD8+ and CD14+ depicted increased pro-apoptotic gene expression after HMG treatment in co-culture. Additionally, HMG treatment increased apoptosis by down-regulating the regulation of anti-apoptotic genes and up-regulating the pro-apoptotic genes in MCF-7. ELISA and multiplex assay reflected increased pro-apoptotic factors, and decreased anti-apoptotic soluble factors, by non-activated and activated CD8+ and CD14+, in a single or co-culture with MCF-7 after HMG treatment. In conclusion, HMG extract possesses immunoadjuvant properties that can be a potential anticancer agent without causing any deleterious effects on the human immune cells.

Full text available from: Official URL: http://www.ifrj.upm.edu.my/30%20(02)%202023/17%20-...

Abstract

The present work investigated the chemopreventive and immunoadjuvant properties of edible bird’s nest (EBN) extract on breast cancer cell line (MCF-7). Specifically, the cytotoxicity level of EBN extracts (HMG, EHMG, pHMG) against MCF-7, human immune cells of cytotoxic T cells, and monocytes (CD8+ and CD14+) were evaluated by measuring the production of pro-apoptotic and anti-apoptotic molecules released in single and co-culture of MCF-7, CD8+, and CD14+ cells, before and after EBN treatment. The highest cytotoxic effect towards MCF-7 using IC50 of 15 µg/mL was demonstrated by HMG but no effects on CD8+ and CD14+, with cell viability of more than 90%. At the mRNA level, activated CD8+ and CD14+ depicted increased pro-apoptotic gene expression after HMG treatment in co-culture. Additionally, HMG treatment increased apoptosis by down-regulating the regulation of anti-apoptotic genes and up-regulating the pro-apoptotic genes in MCF-7. ELISA and multiplex assay reflected increased pro-apoptotic factors, and decreased anti-apoptotic soluble factors, by non-activated and activated CD8+ and CD14+, in a single or co-culture with MCF-7 after HMG treatment. In conclusion, HMG extract possesses immunoadjuvant properties that can be a potential anticancer agent without causing any deleterious effects on the human immune cells.

[error in script]

Item Type:	Article
AGROVOC Term:	bird nests
AGROVOC Term:	neoplasms
AGROVOC Term:	Chemopreventive
AGROVOC Term:	cell culture
AGROVOC Term:	immunostimulation
AGROVOC Term:	immune system
AGROVOC Term:	disease prevention
Geographical Term:	Malaysia
Depositing User:	Nor Hasnita Abdul Samat
Date Deposited:	01 Jul 2025 05:06
Last Modified:	01 Jul 2025 05:06
URI:	http://webagris.upm.edu.my/id/eprint/565

Actions (login required)

View Item