Analytical method for multimycotoxins in roasted coffee samples using liquid chromatography-tandem mass spectrometry

Choi, B and Kim, J. H. and Lee, K. S. and Kim, C. I. and Lee, J. Y. and Park, H. M. (2023) Analytical method for multimycotoxins in roasted coffee samples using liquid chromatography-tandem mass spectrometry. International Food Research Journal (Malaysia), 30. pp. 487-496. ISSN 2231 7546

Mycotoxins are natural toxins that consist of secondary metabolites produced by fungal species of Aspergillus, Fusarium, and Penicillium. The present work aimed to validate the analytical method for detecting multimycotoxins (aflatoxin B1, B2, G1, G2, fumonisin B1, B2, ochratoxin A, and zearalenone) in roasted coffee samples using liquid chromatography-tandem mass spectrometry (LC-MS/MS). Eight stable 13C isotopelabelled internal standards were used for quantification, and an immunoaffinity column (IAC) was used for sample pre-treatment to eliminate interferences. Calibration curves showed good fitness (R2 > 0.995) for all mycotoxins tested. The method detection limit (MDL) and method quantification limit (MQL) for eight mycotoxins were in the range of 0.002 - 0.2 and 0.005 - 0.5 ng/g, respectively. The recoveries ranged from 98.2 to 111% at three concentrations. The coefficients of variation (CVs) ranged from 1.2 to 14% intraday, and 1.4 to 13% interday. These results were within the acceptable range of the Codex Alimentarius Commission (CAC), thus indicating that the validated method could be suitable for multimycotoxin detection in roasted coffee samples.

Full text available from: Official URL: http://www.ifrj.upm.edu.my/30%20(02)%202023/18%20-...

Abstract

Mycotoxins are natural toxins that consist of secondary metabolites produced by fungal species of Aspergillus, Fusarium, and Penicillium. The present work aimed to validate the analytical method for detecting multimycotoxins (aflatoxin B1, B2, G1, G2, fumonisin B1, B2, ochratoxin A, and zearalenone) in roasted coffee samples using liquid chromatography-tandem mass spectrometry (LC-MS/MS). Eight stable 13C isotopelabelled internal standards were used for quantification, and an immunoaffinity column (IAC) was used for sample pre-treatment to eliminate interferences. Calibration curves showed good fitness (R2 > 0.995) for all mycotoxins tested. The method detection limit (MDL) and method quantification limit (MQL) for eight mycotoxins were in the range of 0.002 - 0.2 and 0.005 - 0.5 ng/g, respectively. The recoveries ranged from 98.2 to 111% at three concentrations. The coefficients of variation (CVs) ranged from 1.2 to 14% intraday, and 1.4 to 13% interday. These results were within the acceptable range of the Codex Alimentarius Commission (CAC), thus indicating that the validated method could be suitable for multimycotoxin detection in roasted coffee samples.

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Item Type:	Article
AGROVOC Term:	coffee
AGROVOC Term:	mycotoxins
AGROVOC Term:	food analysis
AGROVOC Term:	gas liquid chromatography
AGROVOC Term:	mass spectrometry
AGROVOC Term:	sampling
AGROVOC Term:	food safety
AGROVOC Term:	food contamination
Geographical Term:	South Korea
Depositing User:	Nor Hasnita Abdul Samat
Date Deposited:	07 Jul 2025 07:45
Last Modified:	07 Jul 2025 07:45
URI:	http://webagris.upm.edu.my/id/eprint/566

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