Estimation of nuclear DNA content of various bamboo and rattan species

Kumar, P. Prakash and Arumuganathan, K. (1999) Estimation of nuclear DNA content of various bamboo and rattan species. [Proceedings Paper]

Nuclear DNA content for a given species is fairly constant between individuals. The paucity of information on the genome size of angiosperms, in particular, of the tropical species including palms, has been pointed out by Bennett and Leitch (1995). We have determined the nuclear DNA content (genome size) of 36 species of bamboo and rattan. The 2C DNA per nucleus was quantified by floww cytometry. The fluorescence of nuclei isolated from the leaves and stained with propidium iodide was measured. This information will be useful for scientists working in diverse areas of plant biology such as biodiversity, plant genome analysis, plant breeding, physiology and molecular biology. Such data may also be utilized to correlate the genome size with the ploidy status of bamb00 species in cases where ploidy status has been reported. Also, if the ploidy of a species is known, a large number of individuals of that species from diverse collections can be screened relatively quickly using this method. This is achievable because the 2C DNA amount per nucleus of a given species and the ploidy status will be directly proportional to each other. To the best of our knowledge, this study is the first of its kind for bamboo and rattan.

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Abstract

Nuclear DNA content for a given species is fairly constant between individuals. The paucity of information on the genome size of angiosperms, in particular, of the tropical species including palms, has been pointed out by Bennett and Leitch (1995). We have determined the nuclear DNA content (genome size) of 36 species of bamboo and rattan. The 2C DNA per nucleus was quantified by floww cytometry. The fluorescence of nuclei isolated from the leaves and stained with propidium iodide was measured. This information will be useful for scientists working in diverse areas of plant biology such as biodiversity, plant genome analysis, plant breeding, physiology and molecular biology. Such data may also be utilized to correlate the genome size with the ploidy status of bamb00 species in cases where ploidy status has been reported. Also, if the ploidy of a species is known, a large number of individuals of that species from diverse collections can be screened relatively quickly using this method. This is achievable because the 2C DNA amount per nucleus of a given species and the ploidy status will be directly proportional to each other. To the best of our knowledge, this study is the first of its kind for bamboo and rattan.

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Item Type:	Proceedings Paper
Additional Information:	Available at Perpustakaan Sultan Abdul Samad, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia. SB317 B2I35 1997 Call Number
AGROVOC Term:	bamboo
AGROVOC Term:	canes and rattans
AGROVOC Term:	plant genetic resources
AGROVOC Term:	protected plant species
AGROVOC Term:	cytogenetics
AGROVOC Term:	taxonomy
AGROVOC Term:	genotyping
AGROVOC Term:	botanists
AGROVOC Term:	genetic variation
Geographical Term:	Singapore
Depositing User:	Nor Hasnita Abdul Samat
Date Deposited:	06 Aug 2025 01:33
Last Modified:	06 Aug 2025 01:33
URI:	http://webagris.upm.edu.my/id/eprint/689

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