A molybdenum-reducing Bacillus sp. Strain zeid 14 in soils from Sudan that could grow on amides and acetonitrile

Ahmad S. A., . and Abu Zeid I. M., . and Abdullah S. R. S., . and Masdor N. A., . and Mohd Adnan A. S., . and Shukor M. S., . and Halmi M. I. E., . and Shukor M. Y., . A molybdenum-reducing Bacillus sp. Strain zeid 14 in soils from Sudan that could grow on amides and acetonitrile. pp. 111-134. ISSN 1394-7990

Agricultural and industrial activities contribute most to pollutants found globally and bioremediation of these pollutants is being intensely sought. We have isolated a molybdenum-reducing bacterium from agricultural soil for bioremediation purposes. The bacterium was grown in low phosphate medium supplemented with molybdate in a microplate format. The molybdenum-reducing bacterium was then further screened for amide-degrading properties. The bacterium was able to use acrylamide as a source of electron donor for reduction and was able to grow on acrylamide acetamide and acetonitrile. The growth parameters obtained according to the modified Gompertz model were lag periods of 0.468 0.979 and 1.53 d and maximum specific growth rates of 1.165 0.932 0.842 d-1 for acrylamide acetamide and acetonitrile respectively. Optimal conditions for molybdate reduction included glucose pH between 6.0 and 6.8 temperature between 25o C and 34o C and phosphate and molybdate concentrations between 5 and 7.5 mM and 10 and 20 mM respectively. The Mo-blue exhibited a unique absorption spectrum closely resembling a reduced phosphomolybdate. Mo-blue production was inhibited by the heavy metals copper mercury silver chromium and cadmium. The bacterium was identified as Bacillus sp. strain Zeid 14. The bacterium will be very useful for bioremediation of sites contaminated with molybdenum and amides.

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Abstract

Agricultural and industrial activities contribute most to pollutants found globally and bioremediation of these pollutants is being intensely sought. We have isolated a molybdenum-reducing bacterium from agricultural soil for bioremediation purposes. The bacterium was grown in low phosphate medium supplemented with molybdate in a microplate format. The molybdenum-reducing bacterium was then further screened for amide-degrading properties. The bacterium was able to use acrylamide as a source of electron donor for reduction and was able to grow on acrylamide acetamide and acetonitrile. The growth parameters obtained according to the modified Gompertz model were lag periods of 0.468 0.979 and 1.53 d and maximum specific growth rates of 1.165 0.932 0.842 d-1 for acrylamide acetamide and acetonitrile respectively. Optimal conditions for molybdate reduction included glucose pH between 6.0 and 6.8 temperature between 25o C and 34o C and phosphate and molybdate concentrations between 5 and 7.5 mM and 10 and 20 mM respectively. The Mo-blue exhibited a unique absorption spectrum closely resembling a reduced phosphomolybdate. Mo-blue production was inhibited by the heavy metals copper mercury silver chromium and cadmium. The bacterium was identified as Bacillus sp. strain Zeid 14. The bacterium will be very useful for bioremediation of sites contaminated with molybdenum and amides.

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Item Type:	Article
AGROVOC Term:	Molybdenum
AGROVOC Term:	Bacillus
AGROVOC Term:	Amides
AGROVOC Term:	Acrylamide
AGROVOC Term:	Agricultural soils
AGROVOC Term:	Isolation
AGROVOC Term:	Identification
AGROVOC Term:	Molybdates
AGROVOC Term:	Mathematical models
AGROVOC Term:	HPLC
Depositing User:	Mr. AFANDI ABDUL MALEK
Last Modified:	24 Apr 2025 00:53
URI:	http://webagris.upm.edu.my/id/eprint/7764

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