Enterococci isolated from traditional cheese alters serum lipid and cytokine levels in adult male NMRI mice

Kalkhorani N., . and Solati J., . Enterococci isolated from traditional cheese alters serum lipid and cytokine levels in adult male NMRI mice. pp. 309-315. ISSN 1394-035X

Introduction: The present study aimed to isolate Enterococci bacteria from Iranian traditional cheese and to evaluate the effects of the isolated bacteria on serum lipid levels and the activation of the immune system in animals. Methods: Enterococci bacteria were isolated from cheese using particular cultures and identified using analytical profile index (API) kits. NMRI mice were divided into different groups and Enterococci was orally administrated at doses of 2 (6108 CFU/ml) 3 (9108 CFU/ml) and 4 (12108 CFU/ml) MacFarland for 2 weeks. Blood samples were collected from a retro-orbital sinus and serum lipids level was measured using the spectrophotometer method and interleukin levels were measured using ELISAkits. Results: Treatment with E.faecium serotype decreased serum total cholesterol and LDL and increased serum IL-10 levels while having no significant effect on serum triglycerides HDL IL-2 and IL-6 concentrations (p 0.05). Administration of E. faecalis serotype had no significant effect on serum lipid levels. Moreover results revealed that treatment with the E. faecalis serotype increased Il-6 and IL-10 concentrations. None of the mentioned serotypes significantly affected gut pathogen growth (p 0.05). Conclusions: Enterococci bacteria from Iranian traditional cheese showed no significant inhibitory effects on gut pathogens but exhibited significant decreases in serum total cholesterol and LDPchol. More studies are suggested to confirm the potential role of the E.faecalis serotype and anti-inflammatory/inflammatory reactions in the body.

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Abstract

Introduction: The present study aimed to isolate Enterococci bacteria from Iranian traditional cheese and to evaluate the effects of the isolated bacteria on serum lipid levels and the activation of the immune system in animals. Methods: Enterococci bacteria were isolated from cheese using particular cultures and identified using analytical profile index (API) kits. NMRI mice were divided into different groups and Enterococci was orally administrated at doses of 2 (6108 CFU/ml) 3 (9108 CFU/ml) and 4 (12108 CFU/ml) MacFarland for 2 weeks. Blood samples were collected from a retro-orbital sinus and serum lipids level was measured using the spectrophotometer method and interleukin levels were measured using ELISAkits. Results: Treatment with E.faecium serotype decreased serum total cholesterol and LDL and increased serum IL-10 levels while having no significant effect on serum triglycerides HDL IL-2 and IL-6 concentrations (p 0.05). Administration of E. faecalis serotype had no significant effect on serum lipid levels. Moreover results revealed that treatment with the E. faecalis serotype increased Il-6 and IL-10 concentrations. None of the mentioned serotypes significantly affected gut pathogen growth (p 0.05). Conclusions: Enterococci bacteria from Iranian traditional cheese showed no significant inhibitory effects on gut pathogens but exhibited significant decreases in serum total cholesterol and LDPchol. More studies are suggested to confirm the potential role of the E.faecalis serotype and anti-inflammatory/inflammatory reactions in the body.

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Item Type:	Article
AGROVOC Term:	Enterococcus
AGROVOC Term:	Enterococcus faecalis
AGROVOC Term:	Enterococcus faecium
AGROVOC Term:	Cheese
AGROVOC Term:	Cytokines
AGROVOC Term:	Mice
AGROVOC Term:	Animal models
AGROVOC Term:	Isolation
AGROVOC Term:	Culture media
AGROVOC Term:	Pathogens
Depositing User:	Mr. AFANDI ABDUL MALEK
Last Modified:	24 Apr 2025 00:53
URI:	http://webagris.upm.edu.my/id/eprint/7772

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