Citation
Salihah N. T., . and Hossain M. M., . and Abdul Hamid M. R. W., . and Ahmed M. U., . A novel rapid and sensitive real-time PCR assay for cost-effective detection and quantification of Staphylococcus aureus in food samples with the ZEN„ doublequenched probe chemistry. pp. 193-201. ISSN 2231-7546
Abstract
Staphylococcus aureus is a prevalent food pathogen. The pathogen is very dangerous as it could survive high cooking temperature and produce heat-stable toxins. Therefore a fast sensitive and secure culture-free alternative to detect and correctly quantify S. aureus in food samples to prevent food poisoning associated with it is needed. In the present work the development of a comparatively inexpensive real-time qPCR assay based on the ZENTM probe chemistry by targeting the thermonuclease gene for a fast sensitive cost-effective and direct quantitative detection of S. aureus in real food samples is reported. The novel assay showed high specificity and sensitivity with the limit of detection (LoD) of as low as 1 copy /reaction. The quantification range varies between 110 and 110 7 copies/reaction. This method was able to accurately quantify S. aureus from milk sample with a relative accuracy of 75.88 to 120. The novel assay successfully detected the presence of as low as 50 copies/mL or copies/g of S. aureus in real food matrices.
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Abstract
Staphylococcus aureus is a prevalent food pathogen. The pathogen is very dangerous as it could survive high cooking temperature and produce heat-stable toxins. Therefore a fast sensitive and secure culture-free alternative to detect and correctly quantify S. aureus in food samples to prevent food poisoning associated with it is needed. In the present work the development of a comparatively inexpensive real-time qPCR assay based on the ZENTM probe chemistry by targeting the thermonuclease gene for a fast sensitive cost-effective and direct quantitative detection of S. aureus in real food samples is reported. The novel assay showed high specificity and sensitivity with the limit of detection (LoD) of as low as 1 copy /reaction. The quantification range varies between 110 and 110 7 copies/reaction. This method was able to accurately quantify S. aureus from milk sample with a relative accuracy of 75.88 to 120. The novel assay successfully detected the presence of as low as 50 copies/mL or copies/g of S. aureus in real food matrices.
Additional Metadata
Item Type: | Article |
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AGROVOC Term: | Staphylococcus aureus |
AGROVOC Term: | Pathogens |
AGROVOC Term: | Identification |
AGROVOC Term: | PCR |
AGROVOC Term: | DNA probes |
AGROVOC Term: | Bioassays |
AGROVOC Term: | Culture media |
AGROVOC Term: | Skimmed milk |
AGROVOC Term: | Bacterial contamination |
AGROVOC Term: | Food contamination |
Depositing User: | Mr. AFANDI ABDUL MALEK |
Last Modified: | 24 Apr 2025 00:53 |
URI: | http://webagris.upm.edu.my/id/eprint/7777 |
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