A novel rapid and sensitive real-time PCR assay for cost-effective detection and quantification of Staphylococcus aureus in food samples with the ZEN„ doublequenched probe chemistry

Salihah N. T., . and Hossain M. M., . and Abdul Hamid M. R. W., . and Ahmed M. U., . A novel rapid and sensitive real-time PCR assay for cost-effective detection and quantification of Staphylococcus aureus in food samples with the ZEN„ doublequenched probe chemistry. pp. 193-201. ISSN 2231-7546

Staphylococcus aureus is a prevalent food pathogen. The pathogen is very dangerous as it could survive high cooking temperature and produce heat-stable toxins. Therefore a fast sensitive and secure culture-free alternative to detect and correctly quantify S. aureus in food samples to prevent food poisoning associated with it is needed. In the present work the development of a comparatively inexpensive real-time qPCR assay based on the ZENTM probe chemistry by targeting the thermonuclease gene for a fast sensitive cost-effective and direct quantitative detection of S. aureus in real food samples is reported. The novel assay showed high specificity and sensitivity with the limit of detection (LoD) of as low as 1 copy /reaction. The quantification range varies between 110 and 110 7 copies/reaction. This method was able to accurately quantify S. aureus from milk sample with a relative accuracy of 75.88 to 120. The novel assay successfully detected the presence of as low as 50 copies/mL or copies/g of S. aureus in real food matrices.

Full text available from: Official URL: http://www.ifrj.upm.edu.my/26%20(01)%202019/(21).p...

Abstract

Staphylococcus aureus is a prevalent food pathogen. The pathogen is very dangerous as it could survive high cooking temperature and produce heat-stable toxins. Therefore a fast sensitive and secure culture-free alternative to detect and correctly quantify S. aureus in food samples to prevent food poisoning associated with it is needed. In the present work the development of a comparatively inexpensive real-time qPCR assay based on the ZENTM probe chemistry by targeting the thermonuclease gene for a fast sensitive cost-effective and direct quantitative detection of S. aureus in real food samples is reported. The novel assay showed high specificity and sensitivity with the limit of detection (LoD) of as low as 1 copy /reaction. The quantification range varies between 110 and 110 7 copies/reaction. This method was able to accurately quantify S. aureus from milk sample with a relative accuracy of 75.88 to 120. The novel assay successfully detected the presence of as low as 50 copies/mL or copies/g of S. aureus in real food matrices.

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Item Type:	Article
AGROVOC Term:	Staphylococcus aureus
AGROVOC Term:	Pathogens
AGROVOC Term:	Identification
AGROVOC Term:	PCR
AGROVOC Term:	DNA probes
AGROVOC Term:	Bioassays
AGROVOC Term:	Culture media
AGROVOC Term:	Skimmed milk
AGROVOC Term:	Bacterial contamination
AGROVOC Term:	Food contamination
Depositing User:	Mr. AFANDI ABDUL MALEK
Last Modified:	24 Apr 2025 00:53
URI:	http://webagris.upm.edu.my/id/eprint/7777

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