Preparation and physicochemical characterization of fish skin gelatine hydrolysate from shortfin scad (Decapterus macrosoma)

H. I. Rasli, . and N. M. Sarbon, . Preparation and physicochemical characterization of fish skin gelatine hydrolysate from shortfin scad (Decapterus macrosoma). pp. 287-294. ISSN 2231-7546

Enzymatic hydrolysis of proteins is an important bioprocess method to prepare bioactive peptides with many functionality and health benefits. The aims of the present work were to prepare and determine the physicochemical characteristics of gelatine hydrolysate from skin of shortfin scad (SSGH) via hydrolysis using alcalase. Analyses on chemical composition molecular weight by SDS PAGE protein concentration amino acid composition Fourier Transform Infrared Spectroscopic features and solubility of SSGH were thus performed. The yield of SSGH obtained was 51.01 (d.b.). The chemical compositions of SSGH for moisture protein fat and ash were 13.82 0.9005 0.0195 and 12.48 respectively. SSGH showed low molecular weight (17 kDa) with a protein concentration of 33.53 mg/mL. Amino acid composition of SSGH contained high amount of glycine proline glutamic acid alanine and arginine with a total content of essential amino acids of 10.74. The solubility of SSG and SSGH were higher at pH 10 and amide I II III A and B were found in both SSG and SSGH.

Full text available from: Official URL: http://www.ifrj.upm.edu.my/26%20(01)%202019/(32).p...

Abstract

Enzymatic hydrolysis of proteins is an important bioprocess method to prepare bioactive peptides with many functionality and health benefits. The aims of the present work were to prepare and determine the physicochemical characteristics of gelatine hydrolysate from skin of shortfin scad (SSGH) via hydrolysis using alcalase. Analyses on chemical composition molecular weight by SDS PAGE protein concentration amino acid composition Fourier Transform Infrared Spectroscopic features and solubility of SSGH were thus performed. The yield of SSGH obtained was 51.01 (d.b.). The chemical compositions of SSGH for moisture protein fat and ash were 13.82 0.9005 0.0195 and 12.48 respectively. SSGH showed low molecular weight (17 kDa) with a protein concentration of 33.53 mg/mL. Amino acid composition of SSGH contained high amount of glycine proline glutamic acid alanine and arginine with a total content of essential amino acids of 10.74. The solubility of SSG and SSGH were higher at pH 10 and amide I II III A and B were found in both SSG and SSGH.

[error in script]

Item Type:	Article
AGROVOC Term:	Decapterus macrosoma
AGROVOC Term:	Skin
AGROVOC Term:	Fish
AGROVOC Term:	Gelatine
AGROVOC Term:	Enzymatic hydrolysis
AGROVOC Term:	Protein hydrolysate
AGROVOC Term:	Physicochemical properties
AGROVOC Term:	Chemical composition
AGROVOC Term:	Molecular weight
AGROVOC Term:	Protein content
Depositing User:	Mr. AFANDI ABDUL MALEK
Last Modified:	24 Apr 2025 00:53
URI:	http://webagris.upm.edu.my/id/eprint/7788

Actions (login required)

View Item