Genetic variation of Clupeonella engrauliformis populations inferred from RFLP analysis of mitochondrial DNA D-loop region on the Southern coast of the Caspian Sea Iran

F. Laloei, . and A. Eimanifar, . and S. Rezvani, . Genetic variation of Clupeonella engrauliformis populations inferred from RFLP analysis of mitochondrial DNA D-loop region on the Southern coast of the Caspian Sea Iran. pp. 929-941. ISSN 0116-6514

Analysis of the genetic structure of the Clupeonella engrauliformis one of the bony fish species with high commercial value was undertaken to gain information regarding the differentiation of this species from two distinct geographical areas (Anzali and Amirabad) located at the western and eastern parts of the Southern Caspian Sea Iran. Restriction fragment length polymorphism (RFLP) analysis was performed on PCR amplified DNA fragments containing the D-loop region of the C. engrauliformis mitochondrial DNA. A total of 100 individuals collected from two areas were surveyed with 18 endonucleases. Six out of 18 enzymes were polymorphic yielding a total of 12 composite haplotypes. Chi-square tests showed significant results in haplotype frequencies among populations and analysis of molecular variance (AMOVA) tests indicated that most of the variation was due to within populations (98.8 ) with FST 0.01172. Despite the existence of significant differentiation between haplotypes for mtDNA variation no consistent pattern of genetic structuring was revealed and nucleotide divergence among C. engrauliformis populations was low. The results indicated that different genotypes exist in these areas resulting in identification of genetic stocks of C. engrauliformis. Therefore careful attention must be given to these matters for the effective cultivation management and protection of C. engrauliformis stock on the Southern coast of the Caspian Sea.

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Abstract

Analysis of the genetic structure of the Clupeonella engrauliformis one of the bony fish species with high commercial value was undertaken to gain information regarding the differentiation of this species from two distinct geographical areas (Anzali and Amirabad) located at the western and eastern parts of the Southern Caspian Sea Iran. Restriction fragment length polymorphism (RFLP) analysis was performed on PCR amplified DNA fragments containing the D-loop region of the C. engrauliformis mitochondrial DNA. A total of 100 individuals collected from two areas were surveyed with 18 endonucleases. Six out of 18 enzymes were polymorphic yielding a total of 12 composite haplotypes. Chi-square tests showed significant results in haplotype frequencies among populations and analysis of molecular variance (AMOVA) tests indicated that most of the variation was due to within populations (98.8 ) with FST 0.01172. Despite the existence of significant differentiation between haplotypes for mtDNA variation no consistent pattern of genetic structuring was revealed and nucleotide divergence among C. engrauliformis populations was low. The results indicated that different genotypes exist in these areas resulting in identification of genetic stocks of C. engrauliformis. Therefore careful attention must be given to these matters for the effective cultivation management and protection of C. engrauliformis stock on the Southern coast of the Caspian Sea.

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Item Type:	Article
AGROVOC Term:	Clupeonella engrauliformis
AGROVOC Term:	Fishes
AGROVOC Term:	Aquatic animals
AGROVOC Term:	Aquatic organisms
AGROVOC Term:	analysis
AGROVOC Term:	DNA
AGROVOC Term:	Enzymes
AGROVOC Term:	Genotypes
AGROVOC Term:	Mitochondria
AGROVOC Term:	Nucleotides
Depositing User:	Mr. AFANDI ABDUL MALEK
Last Modified:	24 Apr 2025 00:53
URI:	http://webagris.upm.edu.my/id/eprint/7841

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