Glucose lowering effect of horseradish tree (Moringa oleifera Lam). leaf decoction in alloxan-induced diabetic mice

Estacio Maria Amelita C., . and Hurtada Wilma A., . and Dizon Erlinda I., . and Ilagan Joan R., . and Barrion Aimee Sheree A., . Glucose lowering effect of horseradish tree (Moringa oleifera Lam). leaf decoction in alloxan-induced diabetic mice. pp. 267-278. ISSN 1394-035X

Introduction: The study was conducted to determine the glucose lowering effect of horseradish tree leaf decoction (HTLD) in alloxan-induced diabetic mice. Methods: The leaves of the Institute of Plant Breeding Moringa 3 (IPBM3) strain of horseradish tree were used in this study. The median effective dose (ED‚‚) of IPBM3 HTLD was determined using normal Institute of Cancer Research (ICR) mice for 14 days and the computed ED‚‚ was administered once twice and thrice per day for 28 days via gavage in alloxan-induced diabetic mice. Fasting blood glucose level (FBGL) body weight feed intake and water intake were determined weekly. Thin layer chromatography and in vitro glucose lowering activity experiments were also done to determine the major polyphenol found in IPBM3 HTL with glucose lowering activity. Results: Administration of the ED‚‚ 417 mg/20 mL/ kgbw once twice and thrice per day for 28 days significantly reduced the mean FBGL of diabetic mice by more than 50 and the reduction was statistically comparable with the metformin-treated group. Mean body weights and feed intakes were normal and statistically comparable for all groups. Mean water intakes were slightly higher than in the non-diabetic control group. The major polyphenol in IPBM3 HTLD was found to be quercetin. The in vitro glucose lowering activity experiment showed that both petroleum ether and water extracts of IPBM3 HTL have glucose lowering activity similar to standard quercetin. Conclusion: The present results show that IPBM3 HTLD possesses glucose lowering activity and offers potential for use in the management of diabetes mellitus.

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Abstract

Introduction: The study was conducted to determine the glucose lowering effect of horseradish tree leaf decoction (HTLD) in alloxan-induced diabetic mice. Methods: The leaves of the Institute of Plant Breeding Moringa 3 (IPBM3) strain of horseradish tree were used in this study. The median effective dose (ED‚‚) of IPBM3 HTLD was determined using normal Institute of Cancer Research (ICR) mice for 14 days and the computed ED‚‚ was administered once twice and thrice per day for 28 days via gavage in alloxan-induced diabetic mice. Fasting blood glucose level (FBGL) body weight feed intake and water intake were determined weekly. Thin layer chromatography and in vitro glucose lowering activity experiments were also done to determine the major polyphenol found in IPBM3 HTL with glucose lowering activity. Results: Administration of the ED‚‚ 417 mg/20 mL/ kgbw once twice and thrice per day for 28 days significantly reduced the mean FBGL of diabetic mice by more than 50 and the reduction was statistically comparable with the metformin-treated group. Mean body weights and feed intakes were normal and statistically comparable for all groups. Mean water intakes were slightly higher than in the non-diabetic control group. The major polyphenol in IPBM3 HTLD was found to be quercetin. The in vitro glucose lowering activity experiment showed that both petroleum ether and water extracts of IPBM3 HTL have glucose lowering activity similar to standard quercetin. Conclusion: The present results show that IPBM3 HTLD possesses glucose lowering activity and offers potential for use in the management of diabetes mellitus.

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Item Type:	Article
AGROVOC Term:	Moringa oleifera
AGROVOC Term:	Armoracia rusticana
AGROVOC Term:	Horseradish (tree)
AGROVOC Term:	Mice
AGROVOC Term:	Human diseases
AGROVOC Term:	Diabetes mellitus
AGROVOC Term:	Blood sugar
AGROVOC Term:	Animal models
AGROVOC Term:	Animal feeding
AGROVOC Term:	Fasting
Depositing User:	Mr. AFANDI ABDUL MALEK
Last Modified:	24 Apr 2025 00:53
URI:	http://webagris.upm.edu.my/id/eprint/7870

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