Effect of punicalagin on human colon cancer Caco-2 cells

Omar Ulfat, . and Aloqbi Akram, . and Yousr Marwa, . and Nazlin K. Howell, . Effect of punicalagin on human colon cancer Caco-2 cells. pp. 125-136. ISSN 1394-035X

Introduction: There is increasing evidence of the presence of antioxidant and antimutagenic properties in some fruit species. Punicalagin extracted from pomegranate is reported to have anti-proliferative activities and able to induce toxicity in colon cancer cells. However the molecular mechanisms involved are still poorly understood. In this study in vitro apoptotic and anti-proliferative activities of punicalagin were investigated in human colon cancer Caco-2 cells. Methods: Cell viability was identified and a morphological change by punicalagin was taken. In addition reactive oxygen species (ROS) annexin V and cell cycle were evaluated by flow cytometer at the tested concentrations of 50 and 75 M. Moreover the effects of punicalagin in Caco-2 cells and normal colon epithelial cell line (HCEC) were compared. The Caspases family was investigated as a marker of apoptosis by western blot. Results: Punicalagin (50 and 75 M) showed toxic effects on Caco-2 cells but not on HCEC with both results being confirmed by morphological studies. In the presence of punicalagin cytoplasmic ROS production decreased indicating antioxidant activity whereas superoxide radicals released from mitochondria increased due to mitochondrial dysfunction. Annexin V and caspase family (9 8 and 3) activation confirmed that cell death occurred via the apoptosis pathway in both concentrations of punicalagin. The cell cycle was arrested in the gap 1 (G1)/synthesis (S) transition phase at the concentrations of punicalagin tested. Conclusion: The in vitro results indicate that further studies are warranted to elucidate the potential role of punicalgin as an anti-cancer agent.

Full text available from: Official URL: http://www.nutriweb.org.my/mjn/publication/22-1/k....

Abstract

Introduction: There is increasing evidence of the presence of antioxidant and antimutagenic properties in some fruit species. Punicalagin extracted from pomegranate is reported to have anti-proliferative activities and able to induce toxicity in colon cancer cells. However the molecular mechanisms involved are still poorly understood. In this study in vitro apoptotic and anti-proliferative activities of punicalagin were investigated in human colon cancer Caco-2 cells. Methods: Cell viability was identified and a morphological change by punicalagin was taken. In addition reactive oxygen species (ROS) annexin V and cell cycle were evaluated by flow cytometer at the tested concentrations of 50 and 75 M. Moreover the effects of punicalagin in Caco-2 cells and normal colon epithelial cell line (HCEC) were compared. The Caspases family was investigated as a marker of apoptosis by western blot. Results: Punicalagin (50 and 75 M) showed toxic effects on Caco-2 cells but not on HCEC with both results being confirmed by morphological studies. In the presence of punicalagin cytoplasmic ROS production decreased indicating antioxidant activity whereas superoxide radicals released from mitochondria increased due to mitochondrial dysfunction. Annexin V and caspase family (9 8 and 3) activation confirmed that cell death occurred via the apoptosis pathway in both concentrations of punicalagin. The cell cycle was arrested in the gap 1 (G1)/synthesis (S) transition phase at the concentrations of punicalagin tested. Conclusion: The in vitro results indicate that further studies are warranted to elucidate the potential role of punicalgin as an anti-cancer agent.

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Item Type:	Article
AGROVOC Term:	Colon
AGROVOC Term:	Cancer (disease)
AGROVOC Term:	Pomegranates
AGROVOC Term:	Antioxidants
AGROVOC Term:	Cell cycle
AGROVOC Term:	Cysteine
AGROVOC Term:	Cytoplasm
AGROVOC Term:	In vitro
AGROVOC Term:	Enzyme activity
AGROVOC Term:	Microscopy
Depositing User:	Mr. AFANDI ABDUL MALEK
Last Modified:	24 Apr 2025 00:53
URI:	http://webagris.upm.edu.my/id/eprint/7885

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