Silymarin supplementation attenuation of the lipopolysaccharide-induced metabolic changes in protein malnourished rats

Alhamdan Adel A., . Silymarin supplementation attenuation of the lipopolysaccharide-induced metabolic changes in protein malnourished rats. pp. 137-151. ISSN 1394-035X

Introduction: Protein malnutrition increases the sensitivity to septic shock by impairing antioxidation and immune response. Based on the potent antioxidant effects of silymarin the putative protective role of silymarin against sepsis-induced oxidative damage in protein malnourished rats was investigated. Methods: Adult male Wistar rats were subjected to protein malnutrition via a low-protein diet (8 protein) with and without silymarin supplementation (30 mg/kg/day) for four weeks and compared to a control group on a 18 protein diet At end of the experiment the animals received intraperitoneal injections (i.p.) of 0.1 mg/ kg lipopolysaccharide (LPS) derived from Esdieridtia coil and decapitation 24 h later. Albumin C-reactive protein (CRP) total protein (TP) alkaline phosphates (AKP ALP) aspartate aminotransferase (AST) alanine aminotransferase (ALT) tumor necrosis factor- (TNF-) interleultine-1 (IL-1) and interleukine-6 (IL-6) levels were measured in serum. In the lungs and liver thiobarbituric acid reactive substances (TBARS) and glutathione (GSH) concentrations superoxide dismutase (SOD) catalase (CAT) and glutathione-s-transferase (GST) activities were measured. Results: oxidative and inflammatory processes were significantly increased by the LPS injection and these changes were to a greater extent in the low-protein dietary group compared to control group. Silymarin supplementation in both dietary groups showed marked inhibition in these activities although the affect was more prominent in the control group compared to the rats fed a low-protein diet. Conclusion: The study showed that silymarin protected against the impairment of antioxidation and immune response in protein malnourished rats particularly in septic shock conditions.

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Abstract

Introduction: Protein malnutrition increases the sensitivity to septic shock by impairing antioxidation and immune response. Based on the potent antioxidant effects of silymarin the putative protective role of silymarin against sepsis-induced oxidative damage in protein malnourished rats was investigated. Methods: Adult male Wistar rats were subjected to protein malnutrition via a low-protein diet (8 protein) with and without silymarin supplementation (30 mg/kg/day) for four weeks and compared to a control group on a 18 protein diet At end of the experiment the animals received intraperitoneal injections (i.p.) of 0.1 mg/ kg lipopolysaccharide (LPS) derived from Esdieridtia coil and decapitation 24 h later. Albumin C-reactive protein (CRP) total protein (TP) alkaline phosphates (AKP ALP) aspartate aminotransferase (AST) alanine aminotransferase (ALT) tumor necrosis factor- (TNF-) interleultine-1 (IL-1) and interleukine-6 (IL-6) levels were measured in serum. In the lungs and liver thiobarbituric acid reactive substances (TBARS) and glutathione (GSH) concentrations superoxide dismutase (SOD) catalase (CAT) and glutathione-s-transferase (GST) activities were measured. Results: oxidative and inflammatory processes were significantly increased by the LPS injection and these changes were to a greater extent in the low-protein dietary group compared to control group. Silymarin supplementation in both dietary groups showed marked inhibition in these activities although the affect was more prominent in the control group compared to the rats fed a low-protein diet. Conclusion: The study showed that silymarin protected against the impairment of antioxidation and immune response in protein malnourished rats particularly in septic shock conditions.

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Item Type:	Article
AGROVOC Term:	Silymarin
AGROVOC Term:	Lipopolysaccharides
AGROVOC Term:	Antioxidants
AGROVOC Term:	Blood proteins
AGROVOC Term:	Blood serum
AGROVOC Term:	Alkaline phosphatase
AGROVOC Term:	Aspartate aminotransferase
AGROVOC Term:	Alanine aminotransferase
AGROVOC Term:	Catalase
AGROVOC Term:	Enzyme activity
Depositing User:	Mr. AFANDI ABDUL MALEK
Last Modified:	24 Apr 2025 00:53
URI:	http://webagris.upm.edu.my/id/eprint/7886

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