Citation
Divya P. R., . and Thomas P. C., . and Chandrika V., . and Paulton M. P., . Genetic characterization of Aeromonas hydrophila using protein profiling and RAPD PCR. pp. 763-771. ISSN 0116-6514
Abstract
Genetic relationship among Aeromonas hydrophila isolates from fish and traditional brackishwater farms were evaluated through Randomly Amplified Polymorphic DNA (RAPD) analysis as well as Sodium Dodecyl Sulphate-Polyacrylamide Gel Electrophoresis (SDS PAGE) profile of cellular proteins. PCR amplification of the DNA from the bacterial isolates using five random primers (OPA-01 3 4 05 and 10) produced 46 amplicons which were scorable as distinct bands on agarose gel electrophoresis. Absolute polymorphism of RAPD profile was evident with a unique pattern for each isolate indicating its usefulness as an ideal tool for evaluation of genetic heterogeneity within the species which are not revealed by other methods like morphological and biochemical characterization and cellular protein profile. Cellular protein profile did not reveal significant polymorphism as all the isolates revealed uniform pattern indicating its usefulness as a tool for species level identification. Four protein bands of molecular size viz. 19.5 kDa 25.6 kDa 29 kDa and 65.6 kDa were shared by all the isolates in the study.
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Abstract
Genetic relationship among Aeromonas hydrophila isolates from fish and traditional brackishwater farms were evaluated through Randomly Amplified Polymorphic DNA (RAPD) analysis as well as Sodium Dodecyl Sulphate-Polyacrylamide Gel Electrophoresis (SDS PAGE) profile of cellular proteins. PCR amplification of the DNA from the bacterial isolates using five random primers (OPA-01 3 4 05 and 10) produced 46 amplicons which were scorable as distinct bands on agarose gel electrophoresis. Absolute polymorphism of RAPD profile was evident with a unique pattern for each isolate indicating its usefulness as an ideal tool for evaluation of genetic heterogeneity within the species which are not revealed by other methods like morphological and biochemical characterization and cellular protein profile. Cellular protein profile did not reveal significant polymorphism as all the isolates revealed uniform pattern indicating its usefulness as a tool for species level identification. Four protein bands of molecular size viz. 19.5 kDa 25.6 kDa 29 kDa and 65.6 kDa were shared by all the isolates in the study.
Additional Metadata
| Item Type: | Article |
|---|---|
| AGROVOC Term: | Aeromonas |
| AGROVOC Term: | Aeromonas hydrophila |
| AGROVOC Term: | Gram negative bacteria |
| AGROVOC Term: | Fishes |
| AGROVOC Term: | Brackish water |
| AGROVOC Term: | DNA |
| AGROVOC Term: | Extraction |
| AGROVOC Term: | Isolation |
| AGROVOC Term: | Random amplified polymorphic DNA |
| AGROVOC Term: | RAPD |
| Depositing User: | Mr. AFANDI ABDUL MALEK |
| Last Modified: | 24 Apr 2025 00:53 |
| URI: | http://webagris.upm.edu.my/id/eprint/7930 |
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