Citation
Ning Xi-Bin, . and Dou Yong, . and Ju Er-Yi, . and Liu Dai-Xin, . Study on rapid detection of Vibrio parahaemolyticus with sea food. pp. 331-336. ISSN 0116-6514
Abstract
Indirect enzyme-linked immunosorbent assay (ELISA) was developed for the rapid detection of V. parahaemolyticus . Specific polyclonal antibody that produced in New Zealand white rabbits were used in the indirect competitive ELISA. The best reaction concentration of antigen and antibody is 10�· CFU/ml and 1: 4 0 respectively and ethe most effective concentration of the secondary HRP-labeled antibody is 1: 1 000. This standardized system was established for detecting V. parahaemolyticus in artificially infected seafood and actual seafood respectively. The detection limit is 10�´ CFU/ml detection time is 8hours; however the accuracy could be improved to 10 CFU/ml after pre-enrichment for 8hours. This system could be of practical application since it was close to the detection result by traditional detection methods.
Download File
Full text available from:
Official URL: http://www.asianfisheriessociety.org/publication/d...
|
Abstract
Indirect enzyme-linked immunosorbent assay (ELISA) was developed for the rapid detection of V. parahaemolyticus . Specific polyclonal antibody that produced in New Zealand white rabbits were used in the indirect competitive ELISA. The best reaction concentration of antigen and antibody is 10�· CFU/ml and 1: 4 0 respectively and ethe most effective concentration of the secondary HRP-labeled antibody is 1: 1 000. This standardized system was established for detecting V. parahaemolyticus in artificially infected seafood and actual seafood respectively. The detection limit is 10�´ CFU/ml detection time is 8hours; however the accuracy could be improved to 10 CFU/ml after pre-enrichment for 8hours. This system could be of practical application since it was close to the detection result by traditional detection methods.
Additional Metadata
| Item Type: | Article |
|---|---|
| AGROVOC Term: | Seafoods |
| AGROVOC Term: | Microbiological contamination |
| AGROVOC Term: | Microbiological analysis |
| AGROVOC Term: | Isolation of microorganisms |
| AGROVOC Term: | ELISA |
| AGROVOC Term: | Indirect ELISA |
| AGROVOC Term: | Vibrio parahaemolyticus |
| AGROVOC Term: | Gram negative bacteria |
| AGROVOC Term: | Bacterial counting |
| AGROVOC Term: | Bacterial contamination |
| Depositing User: | Mr. AFANDI ABDUL MALEK |
| Last Modified: | 24 Apr 2025 00:54 |
| URI: | http://webagris.upm.edu.my/id/eprint/7968 |
Actions (login required)
 |
View Item |
