Study on rapid detection of Vibrio parahaemolyticus with sea food


Citation

Ning Xi-Bin, . and Dou Yong, . and Ju Er-Yi, . and Liu Dai-Xin, . Study on rapid detection of Vibrio parahaemolyticus with sea food. pp. 331-336. ISSN 0116-6514

Abstract

Indirect enzyme-linked immunosorbent assay (ELISA) was developed for the rapid detection of V. parahaemolyticus . Specific polyclonal antibody that produced in New Zealand white rabbits were used in the indirect competitive ELISA. The best reaction concentration of antigen and antibody is 10�· CFU/ml and 1: 4 0 respectively and ethe most effective concentration of the secondary HRP-labeled antibody is 1: 1 000. This standardized system was established for detecting V. parahaemolyticus in artificially infected seafood and actual seafood respectively. The detection limit is 10�´ CFU/ml detection time is 8hours; however the accuracy could be improved to 10 CFU/ml after pre-enrichment for 8hours. This system could be of practical application since it was close to the detection result by traditional detection methods.


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Abstract

Indirect enzyme-linked immunosorbent assay (ELISA) was developed for the rapid detection of V. parahaemolyticus . Specific polyclonal antibody that produced in New Zealand white rabbits were used in the indirect competitive ELISA. The best reaction concentration of antigen and antibody is 10�· CFU/ml and 1: 4 0 respectively and ethe most effective concentration of the secondary HRP-labeled antibody is 1: 1 000. This standardized system was established for detecting V. parahaemolyticus in artificially infected seafood and actual seafood respectively. The detection limit is 10�´ CFU/ml detection time is 8hours; however the accuracy could be improved to 10 CFU/ml after pre-enrichment for 8hours. This system could be of practical application since it was close to the detection result by traditional detection methods.

Additional Metadata

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Item Type: Article
AGROVOC Term: Seafoods
AGROVOC Term: Microbiological contamination
AGROVOC Term: Microbiological analysis
AGROVOC Term: Isolation of microorganisms
AGROVOC Term: ELISA
AGROVOC Term: Indirect ELISA
AGROVOC Term: Vibrio parahaemolyticus
AGROVOC Term: Gram negative bacteria
AGROVOC Term: Bacterial counting
AGROVOC Term: Bacterial contamination
Depositing User: Mr. AFANDI ABDUL MALEK
Last Modified: 24 Apr 2025 00:54
URI: http://webagris.upm.edu.my/id/eprint/7968

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