Pathological genomic and phenotypical characterization of Vibrio parahaemolyticus causative agent of acute hepatopancreatic necrosis disease (AHPND) in Mexico

Lozano-Olvera R., . and Gomez-Gil B., . and Bolanmejia C., . and Aguilar-Rendon K. G., . and Soto-Rodriguez S. A., . and Enciso-Ibarra J., . Pathological genomic and phenotypical characterization of Vibrio parahaemolyticus causative agent of acute hepatopancreatic necrosis disease (AHPND) in Mexico. pp. 102-111. ISSN 0116-6514

Moribund whiteleg shrimp (Penaeus vannamei) affected by acute hepatopancreatic necrosis disease (AHPND) from farms in northwestern Mexico were sampled for pathological analysis. Bacterial isolates were molecularly identified as Vibrio parahaemolyticus (VP) by the presence of the tlh gene. The tdh-negative trh-negative and tlh-positive VP strains were further characterized by enterobacterial repetitive intergenic consensus-polymerase chain reaction (ERIC-PCR). The VP pure strains were used in immersion challenges with shrimp and farmed and challenged shrimp presented the same clinical and pathological signs: lethargy empty gut pale and aqueous hepatopancreas and expanded chromatophores. Using fresh mount histological analysis and bacterial density count three stages of AHNPD (initial acute and terminal) were identified in the affected shrimp. Pathognomonic lesions indicated severe desquamation of tubular epithelial cells of the hepatopancreas. VP had different virulence and was dose dependent. VP strains showed wide tolerance to different environment conditions of temperature salinity and pH and pathogenic and non-pathogenic VP strains from Mexico had similar morphological and physiological characteristics but pathogenic VP strains were most sensitive to nalidixic acid and showed resistance to penicillin. Whole genomic sequence of 22 VP strains from Asia Mexico and South America shows the presence of similar chromosomic pathogenic mechanisms and comparative analysis by multilocus sequence analysis (MLSA) of seven genes clearly showed that most of the isolates are independent strains.

Full text available from: Official URL: http://www.asianfisheriessociety.org/publication/d...

Abstract

Moribund whiteleg shrimp (Penaeus vannamei) affected by acute hepatopancreatic necrosis disease (AHPND) from farms in northwestern Mexico were sampled for pathological analysis. Bacterial isolates were molecularly identified as Vibrio parahaemolyticus (VP) by the presence of the tlh gene. The tdh-negative trh-negative and tlh-positive VP strains were further characterized by enterobacterial repetitive intergenic consensus-polymerase chain reaction (ERIC-PCR). The VP pure strains were used in immersion challenges with shrimp and farmed and challenged shrimp presented the same clinical and pathological signs: lethargy empty gut pale and aqueous hepatopancreas and expanded chromatophores. Using fresh mount histological analysis and bacterial density count three stages of AHNPD (initial acute and terminal) were identified in the affected shrimp. Pathognomonic lesions indicated severe desquamation of tubular epithelial cells of the hepatopancreas. VP had different virulence and was dose dependent. VP strains showed wide tolerance to different environment conditions of temperature salinity and pH and pathogenic and non-pathogenic VP strains from Mexico had similar morphological and physiological characteristics but pathogenic VP strains were most sensitive to nalidixic acid and showed resistance to penicillin. Whole genomic sequence of 22 VP strains from Asia Mexico and South America shows the presence of similar chromosomic pathogenic mechanisms and comparative analysis by multilocus sequence analysis (MLSA) of seven genes clearly showed that most of the isolates are independent strains.

[error in script]

Item Type:	Article
AGROVOC Term:	Prawns and shrimps
AGROVOC Term:	Penaeus vannamei
AGROVOC Term:	Animal pathology
AGROVOC Term:	Isolation of microorganisms
AGROVOC Term:	Vibrio parahaemolyticus
AGROVOC Term:	Vibrio
AGROVOC Term:	Bacteria
AGROVOC Term:	Bacterial diseases
AGROVOC Term:	Genes
AGROVOC Term:	Bacterial counting
Depositing User:	Mr. AFANDI ABDUL MALEK
Last Modified:	24 Apr 2025 00:54
URI:	http://webagris.upm.edu.my/id/eprint/7977

Actions (login required)

View Item