Antioxidant properties of crude and cold ethanol precipitated protein from palm kernel cake (PKC) as potential cosmeceutical agent

Kok A. K. D. X., . and Hon W. M., . and Lai K. S., . and Choi W. C., . and M. Mohamad, . and Yap W. S., . Antioxidant properties of crude and cold ethanol precipitated protein from palm kernel cake (PKC) as potential cosmeceutical agent. pp. 159-164. ISSN 0116-6514

Palm kernel cake (PKC) is a by-product obtained from the production of edible oils using oil palm (Elaeis guineensis). PKC is well-known for its high protein content therefore it was chosen as the target of this research to study its antioxidant properties which is an important criteria in cosmeceutical industry. Our studies showed that the extraction of crude protein at 80C resulted in the highest total phenolic content (TPC) and protein yield. It was further seen that precipitation using 80 cold ethanol following protein extraction at 80C gave the best protein yield of 56.6. The antioxidative activity of this precipitated protein was expressed as IC‚‚ for 1-diphenyl 1-2-picrylhydrazyl (DPPH) radical-scavenging capacity (5170.016 mg ml) 2 2-azino-bisC3-ethylbenzothia zoline-6-sulphonic acid (ABTS) radical-scavenging capacity (0.0470.009 mg ml) and ferrous ion-chelating (FIC) ability (210.13 mg ml). Meanwhile the reducing power and TPC were 0.1560.006 trolox equivalent antioxidant capacity (TEAC) mmol g dry weight (DW) and 25.100.58 gallic acid equivalent (GAE) g g DW respectively. The Pearson correlation test further revealed a significantly moderate to strong positive relationships between antioxidant properties with protein content and antioxidant properties with TPC. Taken together both the crude and precipitated protein obtained from PKC showed substantial amount of antioxidative activities which could be used as sustainable source of antioxidant peptides in enhancing the quality of cosmeceutical products.

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Abstract

Palm kernel cake (PKC) is a by-product obtained from the production of edible oils using oil palm (Elaeis guineensis). PKC is well-known for its high protein content therefore it was chosen as the target of this research to study its antioxidant properties which is an important criteria in cosmeceutical industry. Our studies showed that the extraction of crude protein at 80C resulted in the highest total phenolic content (TPC) and protein yield. It was further seen that precipitation using 80 cold ethanol following protein extraction at 80C gave the best protein yield of 56.6. The antioxidative activity of this precipitated protein was expressed as IC‚‚ for 1-diphenyl 1-2-picrylhydrazyl (DPPH) radical-scavenging capacity (5170.016 mg ml) 2 2-azino-bisC3-ethylbenzothia zoline-6-sulphonic acid (ABTS) radical-scavenging capacity (0.0470.009 mg ml) and ferrous ion-chelating (FIC) ability (210.13 mg ml). Meanwhile the reducing power and TPC were 0.1560.006 trolox equivalent antioxidant capacity (TEAC) mmol g dry weight (DW) and 25.100.58 gallic acid equivalent (GAE) g g DW respectively. The Pearson correlation test further revealed a significantly moderate to strong positive relationships between antioxidant properties with protein content and antioxidant properties with TPC. Taken together both the crude and precipitated protein obtained from PKC showed substantial amount of antioxidative activities which could be used as sustainable source of antioxidant peptides in enhancing the quality of cosmeceutical products.

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Item Type:	Article
AGROVOC Term:	Palm kernel cake
AGROVOC Term:	Palm kernels
AGROVOC Term:	Oilseed cakes
AGROVOC Term:	Kernels
AGROVOC Term:	Elaeis guineensis
AGROVOC Term:	Oil palms
AGROVOC Term:	Byproducts
AGROVOC Term:	Precipitation
AGROVOC Term:	Extraction
AGROVOC Term:	Ethanol
Depositing User:	Mr. AFANDI ABDUL MALEK
Last Modified:	24 Apr 2025 00:54
URI:	http://webagris.upm.edu.my/id/eprint/8010

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