Minimal inhibitory concentration of hygromycin for selecting transformed oil palm embryogenic calli

Nor Fakhrana Iskandar, . and Nurfahisza A. Rahman, . and Omar Abd. Rasid, . and Ahmad Parveez Ghulam Kadir, . Minimal inhibitory concentration of hygromycin for selecting transformed oil palm embryogenic calli. pp. 14-27. ISSN 1511-2780

Determination of optimum concentrations for a selection agent is important in obtaining true transformants during the selection stage. In this study the minimal inhibitory concentrations of hygromycin for four different types of oil palm embryogenic calli (suspension fine yellowish and whitish) that were cultured on proliferation maturation and regeneration media were assessed. The embryogenic calli were cultured on media supplemented with different concentrations of hygromycin. Data on embryogenic callus weight increment was recorded monthly for six months during subculturing. Our results demonstrated that oil palm embryogenic calli cultured on regeneration media (EC) were very sensitive to hygromycin. The growth of embryogenic calli was affected at a relatively low concentration of hygromycin as compared to suspension calli that were cultured on proliferation media (L-1). The regeneration of fine yellowish and whitish embryogenic calli was completely inhibited at 5-6 mg litre-1 of hygromycin. In contrast higher concentration of hygromycin at 10 mg litre-1 is needed to inhibit the proliferation of suspension calli cultured on proliferation media. The hygromycin concentrations determined for each of the embryogenic callus types can be used as a guideline to select true transformants in future oil palm transformation works.

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Abstract

Determination of optimum concentrations for a selection agent is important in obtaining true transformants during the selection stage. In this study the minimal inhibitory concentrations of hygromycin for four different types of oil palm embryogenic calli (suspension fine yellowish and whitish) that were cultured on proliferation maturation and regeneration media were assessed. The embryogenic calli were cultured on media supplemented with different concentrations of hygromycin. Data on embryogenic callus weight increment was recorded monthly for six months during subculturing. Our results demonstrated that oil palm embryogenic calli cultured on regeneration media (EC) were very sensitive to hygromycin. The growth of embryogenic calli was affected at a relatively low concentration of hygromycin as compared to suspension calli that were cultured on proliferation media (L-1). The regeneration of fine yellowish and whitish embryogenic calli was completely inhibited at 5-6 mg litre-1 of hygromycin. In contrast higher concentration of hygromycin at 10 mg litre-1 is needed to inhibit the proliferation of suspension calli cultured on proliferation media. The hygromycin concentrations determined for each of the embryogenic callus types can be used as a guideline to select true transformants in future oil palm transformation works.

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Item Type:	Article
AGROVOC Term:	Elaeis
AGROVOC Term:	Elaeis guineensis
AGROVOC Term:	Oil palms
AGROVOC Term:	Genetic transformation
AGROVOC Term:	Tissue culture
AGROVOC Term:	Callus
AGROVOC Term:	Hygromycin
AGROVOC Term:	Inhibition
AGROVOC Term:	Developmental stages
AGROVOC Term:	Tissue proliferation
Depositing User:	Mr. AFANDI ABDUL MALEK
Last Modified:	24 Apr 2025 00:54
URI:	http://webagris.upm.edu.my/id/eprint/8026

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