Genetic diversity among eight species of willow (Salix spp.) from Iran based on SRAP markers


Citation

Daneshvand Elhameh, . and Rahmani Fatemeh, . and Khodakarimi Ali, . Genetic diversity among eight species of willow (Salix spp.) from Iran based on SRAP markers. pp. 75-85. ISSN 1823-3902

Abstract

This study reports the application of sequence-related amplified polymorphism (SRAP) technique in characterization of 8 species of Salix (spp) from Iran by screening 22 primer combinations (PCs). Twenty two SRAP primer combinations could amplify 116 scorable loci of which 107 bands were polymorphic. The amplified DNA fragments were used for calculation and statistical analysis. The Complete linkage cluster was performed and dendrogram drawn with the help of NTSYS pc 2.02 software which revealed two main clusters and several sub-clusters. This investigation showed that genetic distance was relatively significant among these species. The Jaccard similarity coefficient ranged from 0.18 to 0.55. The results also propose that the SRAP marker is a useful tool for evaluation of genetic diversity and relationships among different Salix species.


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Abstract

This study reports the application of sequence-related amplified polymorphism (SRAP) technique in characterization of 8 species of Salix (spp) from Iran by screening 22 primer combinations (PCs). Twenty two SRAP primer combinations could amplify 116 scorable loci of which 107 bands were polymorphic. The amplified DNA fragments were used for calculation and statistical analysis. The Complete linkage cluster was performed and dendrogram drawn with the help of NTSYS pc 2.02 software which revealed two main clusters and several sub-clusters. This investigation showed that genetic distance was relatively significant among these species. The Jaccard similarity coefficient ranged from 0.18 to 0.55. The results also propose that the SRAP marker is a useful tool for evaluation of genetic diversity and relationships among different Salix species.

Additional Metadata

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Item Type: Article
AGROVOC Term: Salicaceae
AGROVOC Term: Salix
AGROVOC Term: Willow
AGROVOC Term: Polymorphism
AGROVOC Term: Plant extracts
AGROVOC Term: PCR
AGROVOC Term: DNA
AGROVOC Term: Statistical analysis
AGROVOC Term: calculation
AGROVOC Term: Genetic diversity within species
Depositing User: Mr. AFANDI ABDUL MALEK
Last Modified: 24 Apr 2025 00:54
URI: http://webagris.upm.edu.my/id/eprint/8046

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