Citation
Daneshvand Elhameh, . and Rahmani Fatemeh, . and Khodakarimi Ali, . Genetic diversity among eight species of willow (Salix spp.) from Iran based on SRAP markers. pp. 75-85. ISSN 1823-3902
Abstract
This study reports the application of sequence-related amplified polymorphism (SRAP) technique in characterization of 8 species of Salix (spp) from Iran by screening 22 primer combinations (PCs). Twenty two SRAP primer combinations could amplify 116 scorable loci of which 107 bands were polymorphic. The amplified DNA fragments were used for calculation and statistical analysis. The Complete linkage cluster was performed and dendrogram drawn with the help of NTSYS pc 2.02 software which revealed two main clusters and several sub-clusters. This investigation showed that genetic distance was relatively significant among these species. The Jaccard similarity coefficient ranged from 0.18 to 0.55. The results also propose that the SRAP marker is a useful tool for evaluation of genetic diversity and relationships among different Salix species.
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Abstract
This study reports the application of sequence-related amplified polymorphism (SRAP) technique in characterization of 8 species of Salix (spp) from Iran by screening 22 primer combinations (PCs). Twenty two SRAP primer combinations could amplify 116 scorable loci of which 107 bands were polymorphic. The amplified DNA fragments were used for calculation and statistical analysis. The Complete linkage cluster was performed and dendrogram drawn with the help of NTSYS pc 2.02 software which revealed two main clusters and several sub-clusters. This investigation showed that genetic distance was relatively significant among these species. The Jaccard similarity coefficient ranged from 0.18 to 0.55. The results also propose that the SRAP marker is a useful tool for evaluation of genetic diversity and relationships among different Salix species.
Additional Metadata
Item Type: | Article |
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AGROVOC Term: | Salicaceae |
AGROVOC Term: | Salix |
AGROVOC Term: | Willow |
AGROVOC Term: | Polymorphism |
AGROVOC Term: | Plant extracts |
AGROVOC Term: | PCR |
AGROVOC Term: | DNA |
AGROVOC Term: | Statistical analysis |
AGROVOC Term: | calculation |
AGROVOC Term: | Genetic diversity within species |
Depositing User: | Mr. AFANDI ABDUL MALEK |
Last Modified: | 24 Apr 2025 00:54 |
URI: | http://webagris.upm.edu.my/id/eprint/8046 |
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