Anti-Bacillus and nitric oxide inhibitory activities of Malaysian Garcinia mangostana extracts and their major constituents

Karunakaran T., . and I. S. Ismail, . and Ee G. C. L., . Anti-Bacillus and nitric oxide inhibitory activities of Malaysian Garcinia mangostana extracts and their major constituents. pp. 705-711. ISSN 2231-7546

Four types of crude extracts were obtained from the stem bark of Garcinia mangostana from which six xanthone derivatives: -mangostin -mangostin garcinone D mangostenol fuscaxanthone C and dulcisxanthone F were isolated. The structures of these compounds were elucidated and determined using spectroscopic techniques such as MS 1D and 2D NMR. The in vitro anti-Bacillus assay was performed using the crude extracts as well as -mangostin and -mangostin against four Bacillus species; B. subtilis ATCC 6633 B. cereus ATCC 33019 B. megaterium ATCC 14581 and B. pumilus ATCC 14884. The ethyl acetate extract showed strong inhibitory activity against B. subtilis B. cereus B. megaterium and B. pumilus in disc diffusion assay with 10.33 0.44 mm 10.33 0.44 mm 9.00 0.00 mm and 11.33 0.17 mm inhibition zones respectively. Nitric oxide inhibition activities indicated that two major compounds (-mangostin -mangostin) exhibited very significant activity in the inhibition of LPS/IFN- stimulated RAW 264.7 macrophages with IC‚‚ values of 29.81 0.77 and 11.72 1.16 M respectively. The chloroform and ethyl acetate extract of G. mangostana showed very potent activities.

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Abstract

Four types of crude extracts were obtained from the stem bark of Garcinia mangostana from which six xanthone derivatives: -mangostin -mangostin garcinone D mangostenol fuscaxanthone C and dulcisxanthone F were isolated. The structures of these compounds were elucidated and determined using spectroscopic techniques such as MS 1D and 2D NMR. The in vitro anti-Bacillus assay was performed using the crude extracts as well as -mangostin and -mangostin against four Bacillus species; B. subtilis ATCC 6633 B. cereus ATCC 33019 B. megaterium ATCC 14581 and B. pumilus ATCC 14884. The ethyl acetate extract showed strong inhibitory activity against B. subtilis B. cereus B. megaterium and B. pumilus in disc diffusion assay with 10.33 0.44 mm 10.33 0.44 mm 9.00 0.00 mm and 11.33 0.17 mm inhibition zones respectively. Nitric oxide inhibition activities indicated that two major compounds (-mangostin -mangostin) exhibited very significant activity in the inhibition of LPS/IFN- stimulated RAW 264.7 macrophages with IC‚‚ values of 29.81 0.77 and 11.72 1.16 M respectively. The chloroform and ethyl acetate extract of G. mangostana showed very potent activities.

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Item Type:	Article
AGROVOC Term:	Garcinia
AGROVOC Term:	Garcinia mangostana
AGROVOC Term:	Mangosteen
AGROVOC Term:	Plant extracts
AGROVOC Term:	Bark extracts
AGROVOC Term:	Isolation in chemical analysis
AGROVOC Term:	Spectroscopy
AGROVOC Term:	Chemical compounds
AGROVOC Term:	Chemical structure
AGROVOC Term:	Chloroform
Depositing User:	Mr. AFANDI ABDUL MALEK
Last Modified:	24 Apr 2025 00:54
URI:	http://webagris.upm.edu.my/id/eprint/8096

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