Enzymatic production of xylooligosaccharides from beechwood xylan: effect of xylanase preparation on carbohydrate profile of the hydrolysates

Guido E. S., . and Silveira J. T., . and Kalil S. J., . Enzymatic production of xylooligosaccharides from beechwood xylan: effect of xylanase preparation on carbohydrate profile of the hydrolysates. pp. 713-721. ISSN 2231-7546

Xylooligosaccharides (XOS) are non-digestible food ingredients with beneficial effects on human health. In the present work three different xylanase preparations from filamentous fungi were characterised and used in the enzymatic conversion of beechwood xylan into XOS. The degree of polymerisation (DP) and yield of XOS besides the release of xylose in the hydrolysate are influenced by the xylanolytic activities of xylanase preparation and biochemical properties of endo-1 4--xylanases. Enzymatic hydrolysis of beechwood xylan using -xylosidase-free xylanase from Thermomyces lanuginosus produced more than 90 (w/v) XOS in the hydrolysate equivalent to 9.18 mg/mL in 24 h of reaction time. The XOS obtained in highest amounts were xylobiose (DP 2 66.46) followed by xylotriose (DP 3 25.10) and a small amount of xylose (4.97) was simultaneously obtained. These findings suggest that this type of xylanase preparation is very promising for obtaining high-pure XOS with lower DP in food industries.

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Abstract

Xylooligosaccharides (XOS) are non-digestible food ingredients with beneficial effects on human health. In the present work three different xylanase preparations from filamentous fungi were characterised and used in the enzymatic conversion of beechwood xylan into XOS. The degree of polymerisation (DP) and yield of XOS besides the release of xylose in the hydrolysate are influenced by the xylanolytic activities of xylanase preparation and biochemical properties of endo-1 4--xylanases. Enzymatic hydrolysis of beechwood xylan using -xylosidase-free xylanase from Thermomyces lanuginosus produced more than 90 (w/v) XOS in the hydrolysate equivalent to 9.18 mg/mL in 24 h of reaction time. The XOS obtained in highest amounts were xylobiose (DP 2 66.46) followed by xylotriose (DP 3 25.10) and a small amount of xylose (4.97) was simultaneously obtained. These findings suggest that this type of xylanase preparation is very promising for obtaining high-pure XOS with lower DP in food industries.

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Item Type:	Article
AGROVOC Term:	Food ingredients
AGROVOC Term:	Oligosaccharides
AGROVOC Term:	Enzymatic hydrolysis
AGROVOC Term:	Enzyme preparations
AGROVOC Term:	Fungi
AGROVOC Term:	Xylans
AGROVOC Term:	Xylose
AGROVOC Term:	Enzymatic activity
AGROVOC Term:	Biochemistry
Depositing User:	Mr. AFANDI ABDUL MALEK
Last Modified:	24 Apr 2025 00:54
URI:	http://webagris.upm.edu.my/id/eprint/8097

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