Chemical components and antibacterial activities of essential oil of wild in vitro and acclimatised plants of Ziziphora tenuior L.

Dakah A., . and Zaid S., . and Suleiman M., . and Dakka M., . Chemical components and antibacterial activities of essential oil of wild in vitro and acclimatised plants of Ziziphora tenuior L. pp. 723-730. ISSN 2231-7546

Ziziphora tenuior is widely used in traditional medicine for the treatment of various diseases. Therefore medicinal plants such as this require continuous investigation to obtain the beneficial compounds they might possess. The essential oils of wild in vitro and acclimatised Z. Tenuior were identified by GC/MS analysis. The compounds in the essential oils were determined by co-injection of the sample with a solution containing the homologous series as standard of C9 to C22 n-alkanes. The activity of the essential oils was evaluated against five bacterial strains by disc diffusion and the MIC was determined based on a micro-well plate (96 well) dilution method. To obtain the MBC 10 L was taken from each well and inoculated on MllerHinton agar. Seventeen volatile compounds were determined the main one being pulegone (45.02-46.43) followed by bicyclo3.1.1hept-3-en-2-one 4 6 6-trimethyl- (12.96- 14.68) and bicyclo2.2.1heptan-2-ol 1 5 5-trimethyl- (11.62-13.18). Two compounds; copaene and -cubebene were only isolated from the essential oil of in vitro plants. The chemical compositions and their percentages differed based on the source of plants. The results obtained demonstrated the influence of plant tissue culture media on the resulting essential oils. The essential oil of all plants showed various antimicrobial activities against Esherichia coli Klebsiella pneumoniae Salmonella Typhi and Staphylococcus aureus with diameters of inhibition zone ranging between 8 and 42 mm while none was observed on Pseudomonas aeruginosa. The largest inhibition zone (42 mm) was observed from the essential oil of in vitro plants (100) against Staph. aureus. The MIC ranged between 0.25 to 2 while MBC between 0.5 to 8 g/mL. It seems that plant tissue culture will be a useful method to modify the chemical composition and their concentration of Z. tenuior essential oil like pulegone and n-hexadecanoic acid. The different chemical compositions are likely to be the result of genetic differences and/or the effect of some plant growth regulators.

Full text available from: Official URL: http://www.ifrj.upm.edu.my/26%20(02)%202019/(39).p...

Abstract

Ziziphora tenuior is widely used in traditional medicine for the treatment of various diseases. Therefore medicinal plants such as this require continuous investigation to obtain the beneficial compounds they might possess. The essential oils of wild in vitro and acclimatised Z. Tenuior were identified by GC/MS analysis. The compounds in the essential oils were determined by co-injection of the sample with a solution containing the homologous series as standard of C9 to C22 n-alkanes. The activity of the essential oils was evaluated against five bacterial strains by disc diffusion and the MIC was determined based on a micro-well plate (96 well) dilution method. To obtain the MBC 10 L was taken from each well and inoculated on MllerHinton agar. Seventeen volatile compounds were determined the main one being pulegone (45.02-46.43) followed by bicyclo3.1.1hept-3-en-2-one 4 6 6-trimethyl- (12.96- 14.68) and bicyclo2.2.1heptan-2-ol 1 5 5-trimethyl- (11.62-13.18). Two compounds; copaene and -cubebene were only isolated from the essential oil of in vitro plants. The chemical compositions and their percentages differed based on the source of plants. The results obtained demonstrated the influence of plant tissue culture media on the resulting essential oils. The essential oil of all plants showed various antimicrobial activities against Esherichia coli Klebsiella pneumoniae Salmonella Typhi and Staphylococcus aureus with diameters of inhibition zone ranging between 8 and 42 mm while none was observed on Pseudomonas aeruginosa. The largest inhibition zone (42 mm) was observed from the essential oil of in vitro plants (100) against Staph. aureus. The MIC ranged between 0.25 to 2 while MBC between 0.5 to 8 g/mL. It seems that plant tissue culture will be a useful method to modify the chemical composition and their concentration of Z. tenuior essential oil like pulegone and n-hexadecanoic acid. The different chemical compositions are likely to be the result of genetic differences and/or the effect of some plant growth regulators.

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Item Type:	Article
AGROVOC Term:	Lamiaceae
AGROVOC Term:	Medicinal plants
AGROVOC Term:	Essential oils
AGROVOC Term:	Determination of chemicals
AGROVOC Term:	Gas chromatography
AGROVOC Term:	Mass spectrometry
AGROVOC Term:	Wild plants
AGROVOC Term:	In vitro culture
AGROVOC Term:	Chemical composition
AGROVOC Term:	Bacteria
Depositing User:	Mr. AFANDI ABDUL MALEK
Last Modified:	24 Apr 2025 00:54
URI:	http://webagris.upm.edu.my/id/eprint/8098

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