Elateriospermum tapos seed protein as a new potential therapeutic for diabetes obesity and hypertension: Extraction and characterization of protein

Naufal Arshad, . and Chee Yuen Gan, . Elateriospermum tapos seed protein as a new potential therapeutic for diabetes obesity and hypertension: Extraction and characterization of protein. pp. 27-43. ISSN 1511-3701

The Elateriospermum tapos seed extract is one of the major contributors to the therapeutic effects as remedy drink for chronic diseases such as hypertension diabetes and obesity by the locals in East Malaysia. It is believed that the component in the seed such as protein plays an essential role due to its nutraceutical properties. Hence we report the extraction of protein from the seed of E. tapos that has the potential biological activities. The protein was initially extracted using salt solution which was similar to the local practice and maximal values of extraction yield (44 mg/g) was performed based on the extraction parameters; pH 8 sample to buffer ratio 1:50 (w/v) 2 h at 80 C. Sodium dedocyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) profile showed that 7S globulin was the dominant protein in the E. tapos seed protein isolate (ETSPI) whereas Fourier Transform Infrared (FTIR) analysis showed that the extracted protein consisted of -helical secondary structure. This protein could be digested up to 72.5 in vitro. In the biological activity evaluation the results showed that ETSPI exhibited angiotensin converting enzyme (ACE) inhibitory (56.6/100 g) lipase inhibitory (26.7/100 g) -amylase inhibitory (31.2/100 g) free radical scavenging (33.4/mg) and metal reducing activities (0.97 mM FeSO4/mg) which suggests that this protein has the potential in the aforementioned therapeutic effects.

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Abstract

The Elateriospermum tapos seed extract is one of the major contributors to the therapeutic effects as remedy drink for chronic diseases such as hypertension diabetes and obesity by the locals in East Malaysia. It is believed that the component in the seed such as protein plays an essential role due to its nutraceutical properties. Hence we report the extraction of protein from the seed of E. tapos that has the potential biological activities. The protein was initially extracted using salt solution which was similar to the local practice and maximal values of extraction yield (44 mg/g) was performed based on the extraction parameters; pH 8 sample to buffer ratio 1:50 (w/v) 2 h at 80 C. Sodium dedocyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) profile showed that 7S globulin was the dominant protein in the E. tapos seed protein isolate (ETSPI) whereas Fourier Transform Infrared (FTIR) analysis showed that the extracted protein consisted of -helical secondary structure. This protein could be digested up to 72.5 in vitro. In the biological activity evaluation the results showed that ETSPI exhibited angiotensin converting enzyme (ACE) inhibitory (56.6/100 g) lipase inhibitory (26.7/100 g) -amylase inhibitory (31.2/100 g) free radical scavenging (33.4/mg) and metal reducing activities (0.97 mM FeSO4/mg) which suggests that this protein has the potential in the aforementioned therapeutic effects.

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Item Type:	Article
AGROVOC Term:	Seeds
AGROVOC Term:	Oilseeds
AGROVOC Term:	Extraction
AGROVOC Term:	Oilseed protein
AGROVOC Term:	Proteins
AGROVOC Term:	Angiotensin
AGROVOC Term:	Enzyme inhibitors
AGROVOC Term:	Lipases
AGROVOC Term:	Alpha amylase
AGROVOC Term:	Therapeutic substances
Depositing User:	Mr. AFANDI ABDUL MALEK
Last Modified:	24 Apr 2025 00:54
URI:	http://webagris.upm.edu.my/id/eprint/8121

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